环氧树脂切片氧化对苯二胺染色。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-07-01 DOI:10.1515/znc-1984-7-824

J C Stockert, R Armas-Portela, O D Colman, J M Ferrer, A Tato

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引用次数: 1

摘要

用氧化对苯二胺(PPD)溶液处理戊二醛固定、环氧树脂包埋的动物组织的半薄切片和薄片。该方法适用于光镜下组织的一般形态显示，对某些含聚阴离子成分的染色程度较高。用氯化金溶液后处理薄片在PPD正极结构中具有相当大的电子不透明性。

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Oxidized p-phenylenediamine staining of epoxy resin sections.

Semithin and thin sections of glutaraldehyde-fixed, epoxy resin-embedded animal tissues were treated with solutions of oxidized p-phenylenediamine (PPD). This method is suitable to reveal the general morphology of tissues in light microscopy, showing a high staining degree in some polyanion containing components. Posttreatments of thin sections with gold chloride solutions give considerable electron opacity in PPD positive structures.

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Zeitschrift fur Naturforschung. Section C, Biosciences

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