【主要胺类局麻药的气液色谱快速同时测定法】。

G Desch, D Cavadore, Y Jullien, L Mercier, B Descomps, M de Rodez
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引用次数: 0

摘要

本方法可在300微升血浆中同时测定术后硬膜外注射用于麻醉和镇痛的三种主要局麻药:xylocaïne、etidocaïne、bupivacaïne。测定方法包括三个步骤:(a)加入内校准剂(mepivacaïne)。(b)用三氯辛酸排便。(c)上清(pH值11)碱化,二氯甲烷萃取,室温下有机相浓缩。(d)用SE 30或OV 17浸渍柱进行色谱分析。该方法在0.37摩尔/ l-1(0.1微克)之间敏感。浓度在0.37摩尔1-1和75摩尔1-1(0.1微克)之间,平均偏差系数为10.9%。Ml-1和20微克。ml-1)。在这个大的浓度范围内记录的数据的对应性没有任何技术上的改变,这意味着可以跟踪硬膜外注射前后血浆浓度的动力学。用气液色谱法测定lidocaïne得到的结果在115个不同的血浆样品中进行了比较,这些血浆样品的浓度由配备在离心机分析仪上的免疫酶法(“EMIT”)获得。两种方法的相关系数为:(r = 0.95, y = 0.09 x +0.25微克。Ml-1意味着没有任何干扰和两种方法的特异性。色谱柱还在20分钟内分离lidocaïne的两种主要代谢物:单乙基甘氨酸乙酯(M.E.G.X.)和甘氨酸乙酯(G.X.)。结果表明,持续硬膜外注射lidocaïne可产生高血药浓度,无临床毒性现象。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Rapid simultaneous assay of the principalamide-type local anesthetics by gas-liquid chromatography].

This method can assay simultaneously, using 300 microliters of plasma, of the three principle local anesthetic agents used by peridural injection for post-operative anesthesia and analgesia: xylocaïne, etidocaïne, bupivacaïne. The assay method consists of three steps: (a) the addition of an internal calibrating agent (mepivacaïne). (b) defecation using trichlorocetic acid. (c) alcalinization of the supernatent (pH 11), extraction with dichloromethane and concentration at room temperature of the organic phase. (d) chromotography using an SE 30 or OV 17 impregnated column. The method is sensitive between 0.37 mumoles per l-1 (0.1 microgram . ml-1) and the coefficient for the mean deviation is 10.9% for concentration between 0.37 mumoles 1-1 and 75 mumole1-1 (0.1 microgram . ml-1 and 20 micrograms . ml-1). The correspondence of the figures recorded in this large concentration range without any change in the technique means that the kinetics of the plasma concentrations before and after peridural injection can be followed. The results obtained by gas liquid chromatography for the assay of lidocaïne were compared in 115 different plasma samples with concentrations obtained by an immuno enzymatic method ("EMIT") fitted to a centrifuge analyser. The correlation coefficient between the two methods was: (r = 0.95 with y = 0.09 x +0.25 microgram . ml-1 implying the absence of any interference and the specificity of the two methods. The columns also separate in 20 minutes the two main metabolites of lidocaïne: monoethylglycinexylidide (M.E.G.X.) and glycinexylidide (G.X.). These results demonstrate that continuous peridural injection of lidocaïne produces a high plasma concentration without any clinical toxic phenomena.

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