{"title":"硫酸层酰胺对培养主动脉细胞脂质合成的抑制作用","authors":"K. Murata","doi":"10.1016/S0368-1319(69)80040-2","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>(1)</span><span><p>Sodium [1-<sup>14</sup>C]acetate was sextensively incorporated into the lipid fractions of chick aortic cells in secondary cultures maintained as monolayers, indicating a linear relation between the incorporation rate and the incubation time over 8 h.</p></span></li><li><span>(2)</span><span><p>When laminaran sulfate was added to this culture system, at concentrations between 2.5 and 40 μg/ml culture medium for 24–72 h, it depressed lipid synthesis in aortic cells, as measured by [<sup>14</sup>C]acetate incorporation rates into the lipid fraction.</p></span></li><li><span>(3)</span><span><p>Inhibition of lipid synthesis depended on the concentration of laminaran sulfate and its incubation time; inhibition was greater in the neutral fat and free sterol fractions than in the phospholipid fraction.</p></span></li></ul></div>","PeriodicalId":78351,"journal":{"name":"Journal of atherosclerosis research","volume":"10 3","pages":"Pages 371-378"},"PeriodicalIF":0.0000,"publicationDate":"1969-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0368-1319(69)80040-2","citationCount":"3","resultStr":"{\"title\":\"Suppression of lipid synthesis in cultured aortic cells by laminaran sulfate\",\"authors\":\"K. Murata\",\"doi\":\"10.1016/S0368-1319(69)80040-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>(1)</span><span><p>Sodium [1-<sup>14</sup>C]acetate was sextensively incorporated into the lipid fractions of chick aortic cells in secondary cultures maintained as monolayers, indicating a linear relation between the incorporation rate and the incubation time over 8 h.</p></span></li><li><span>(2)</span><span><p>When laminaran sulfate was added to this culture system, at concentrations between 2.5 and 40 μg/ml culture medium for 24–72 h, it depressed lipid synthesis in aortic cells, as measured by [<sup>14</sup>C]acetate incorporation rates into the lipid fraction.</p></span></li><li><span>(3)</span><span><p>Inhibition of lipid synthesis depended on the concentration of laminaran sulfate and its incubation time; inhibition was greater in the neutral fat and free sterol fractions than in the phospholipid fraction.</p></span></li></ul></div>\",\"PeriodicalId\":78351,\"journal\":{\"name\":\"Journal of atherosclerosis research\",\"volume\":\"10 3\",\"pages\":\"Pages 371-378\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1969-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0368-1319(69)80040-2\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of atherosclerosis research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0368131969800402\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of atherosclerosis research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0368131969800402","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Suppression of lipid synthesis in cultured aortic cells by laminaran sulfate
(1)
Sodium [1-14C]acetate was sextensively incorporated into the lipid fractions of chick aortic cells in secondary cultures maintained as monolayers, indicating a linear relation between the incorporation rate and the incubation time over 8 h.
(2)
When laminaran sulfate was added to this culture system, at concentrations between 2.5 and 40 μg/ml culture medium for 24–72 h, it depressed lipid synthesis in aortic cells, as measured by [14C]acetate incorporation rates into the lipid fraction.
(3)
Inhibition of lipid synthesis depended on the concentration of laminaran sulfate and its incubation time; inhibition was greater in the neutral fat and free sterol fractions than in the phospholipid fraction.
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