用NIR-II光触发凝胶递送Mir-196c-3p可减轻心肌缺血-再灌注损伤中的心肌细胞铁凋亡

IF 4.7 4区 医学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jing-jing Ji PhD , Shang-yu Chen PhD , Zi-wei Yang MSc , Rui Zhang PhD , Ling-lin Qian PhD , Yu Jiang PhD , Jia-qi Guo PhD , Ya Wu PhD , Qu-li Fan PhD , Yu-yu Yao PhD , Peng-fei Sun PhD
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引用次数: 4

摘要

铁下垂在缺血再灌注(I/R)诱导的心脏损伤中起重要作用,目前靶向递送mirna治疗铁下垂存在许多缺陷。我们在此报道了一种独特的水凝胶(Gel),它可以通过近红外ii (NIR-II)光触发,具有深层组织穿透性和生物相容性最大允许暴露(MPE)值,用于I/R后的原位处理。将mir-196c-3p模拟物(mimics)和光热纳米颗粒(BTN)共封装在具有NIR-II光触发释放的可注射凝胶(mimics + Gel/BTN)中。利用1064 nm光照射,实现了局部微环境光热触发的miRNA按需无创可控递送,旨在抑制I/ r诱导的铁凋亡。因此,通过在I/R模型中控制释放Gel/BTN模拟物,同时抑制铁下垂中心基因NOX4、P53和LOX的表达,可以降低心肌细胞的铁下垂,改善心脏功能,提高大鼠的存活率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Delivery of Mir-196c-3p with NIR-II light-triggered gel attenuates cardiomyocyte ferroptosis in cardiac ischemia-reperfusion injury

Delivery of Mir-196c-3p with NIR-II light-triggered gel attenuates cardiomyocyte ferroptosis in cardiac ischemia-reperfusion injury

Ferroptosis plays an important role in ischemia-reperfusion (I/R)-induced cardiac injury and there are many defects in current targeted delivery of miRNAs for the treatment of ferroptosis. We herein report a unique hydrogel (Gel) that can be triggered by a near-infrared-II (NIR-II) light with deep tissue penetration and biocompatible maximum permissible exposure (MPE) value for in situ treatment after I/R. The mir-196c-3p mimic (mimics) and photothermal nanoparticles (BTN) were co-encapsulated in an injectable Gel (mimics + Gel/BTN) with NIR-II light-triggered release. Using 1064 nm light irradiation, local microenvironment photothermal-triggered on-demand noninvasive controllable delivery of miRNA was achieved, aiming to inhibit I/R-induced ferroptosis. Consequently, declined ferroptosis in cardiomyocytes and improved cardiac function, survival rate in rats was achieved through the controlled release of Gel/BTN mimics in I/R model to simultaneously inhibit ferroptosis hub genes NOX4, P53, and LOX expression.

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来源期刊
CiteScore
8.10
自引率
3.60%
发文量
104
审稿时长
4.6 months
期刊介绍: Nanomedicine: Nanotechnology, Biology and Medicine (NBM) is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
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