紫外线辐射诱导大鼠主动脉张力和磷酸化酶a形成降低。

T M Lincoln, J Laks, R M Johnson
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引用次数: 0

摘要

用去甲肾上腺素(10 nM)收缩的大鼠主动脉螺旋条对紫外线(340-360 nM)辐射有短暂的张力下降。紫外线辐射依赖性松弛是完全可逆的,与内皮细胞无关。虽然张力降低与组织cGMP含量升高有关,但紫外线照射后cAMP水平不变。与cGMP升高和张力下降同时发生的磷酸化酶A形成的显著抑制在紫外线辐射下被观察到。紫外辐射对cGMP、磷酸化酶a形成和张力的影响被亚甲基蓝阻断。更高浓度的去甲肾上腺素(100 nM)也能部分逆转紫外线辐射下磷酸化酶a形成的松弛和抑制。我们的研究结果表明,cGMP可能通过减少细胞质Ca2+介导紫外线辐射依赖性反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ultraviolet radiation-induced decreases in tension and phosphorylase a formation in rat aorta.

Helical strips of rat aorta contracted with norepinephrine (10 nM) respond to ultraviolet (UV) radiation (340-360 nm) with a transient decrease in tension. The UV radiation-dependent relaxation is completely reversible and endothelial cell-independent. Although decreased tension is associated with a rise in tissue cGMP content, the cAMP level is unchanged after UV radiation. A significant inhibition of phosphorylase a formation which occurs coincidently with the rise in cGMP and decline in tension is observed with UV radiation. The effects of UV radiation on cGMP, phosphorylase a formation and tension were blocked by methylene blue. Relaxation and inhibition of phosphorylase a formation in response to UV radiation were also partially reversed with higher concentrations of norepinephrine (100 nM). Our results suggest that cGMP may mediate UV radiation-dependent reactions by reducing cytoplasmic Ca2+.

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