2020年泰国非洲马病暴发期间库蠓（双翅目：蠓科）监测：物种鉴定和非洲马病病毒检测

IF 1.6 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2026-10-01 Epub Date: 2026-10-08 DOI:10.1016/j.vprsr.2026.101605

Rungrat Saiyasombat, Juntra Wattanamethanont, Kittichai Unjit, Montakan Jiratanh, Arunrat Thepparat

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引用次数: 0

摘要

在2020年泰国非洲马病暴发期间，开展了库蠓病媒监测，以确定与疾病传播相关的库蠓种类。在17个受影响地点和2个接种地点设置了紫外线诱蚊灯，收集库蠓。共采集库蠓28226只，经形态学鉴定隶属于1个种群6个亚属15种。其中以oxystoma丰度最高（37.37%），其次为主要媒介imicola C.（24.09%）和innoxius C.（15%）。线粒体细胞色素氧化酶亚基I基因（COI）的DNA条形码鉴定证实了11个物种，其中包括一个额外的物种C. similis。实时RT-PCR检测了280个样本库中的8个样本库（6个样本库为斑胸草库，2个样本库为斑胸草库）的AHS病毒RNA。本研究结合形态学和分子（COI）鉴定，对2020年泰国非洲马病暴发期间库蠓的物种组成和丰度进行了全面、多地点的表征。据我们所知，这是泰国首次对与斑马相关的库蠓进行昆虫学调查，并报道了该国马场收集的库蠓的第一个COI参考序列。

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Surveillance of Culicoides (Diptera: Ceratopogonidae) during the 2020 African horse sickness outbreak in Thailand: Species identification and African horse sickness virus detection

During African horse sickness (AHS) outbreak in Thailand in 2020, Culicoides vector surveillance was conducted to determine Culicoides species associated with the disease transmission. Ultraviolet light traps were set up to collect Culicoides midges at 17 affected and 2 vaccinated sites. A total of 28,226 Culicoides were collected and morphologically identified into 15 species from 6 subgenera and 1 species group. The most abundance species was C. oxystoma (37.37%), followed by C. imicola (24.09%), the main vector of AHS, and C. innoxius (15%). Molecular identification by DNA barcoding of mitochondrial cytochrome oxidase subunit I gene (COI) confirmed 11 species, including an additional species, C. similis. AHS virus RNA was detected by real-time RT-PCR in 8 of 280 pools tested (6 pools of C. oxystoma and 2 pools of C. imicola collected from zebra farms). This study provides a comprehensive, multi-site characterization of Culicoides species composition and abundance during the 2020 African horse sickness outbreak in Thailand, combining morphological and molecular (COI) identification. To the best of our knowledge, this represents the first entomological survey of Culicoides species associated with zebra in Thailand, and additionally reports the first COI reference sequence for C. similis collected from horse farms in this country.

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).