N. Azri-Shah Norhan, Nor Asma Husna Yusoff, Farizan Abdullah, Ahmad Najmi Ishak, Norainy Mohd Husin, Hin Boo Wee, John Brian Jones, Mhd Ikhwanuddin, Siti Azizah Mohd Nor, Marina Hassan
{"title":"马来西亚入侵方头螯虾与本地罗氏沼虾之间的宿主相容性实验研究。","authors":"N. Azri-Shah Norhan, Nor Asma Husna Yusoff, Farizan Abdullah, Ahmad Najmi Ishak, Norainy Mohd Husin, Hin Boo Wee, John Brian Jones, Mhd Ikhwanuddin, Siti Azizah Mohd Nor, Marina Hassan","doi":"10.1007/s11686-026-01415-w","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The introduction of host-associated symbionts by invasive species threatens native aquatic biodiversity through symbiont spillover and host switching. This study investigated the experimental host-specificity and transmission potential of <i>Diceratocephala boschmai</i> from invasive <i>Cherax quadricarinatus</i> to the native giant freshwater prawn <i>Macrobrachium rosenbergii</i>.</p><h3>Methods</h3><p>Briefly, temnocephalid-infested <i>C. quadricarinatus</i> were exposed to symbiont-free <i>M. rosenbergii</i> and <i>C. quadricarinatus</i> for 30 days. Thereafter, symbiont transmission was assessed, and host hemolymph parameters, including total hemocyte count (THC), differential hemocyte count (DHC), glucose, and protein concentration, were evaluated.</p><h3>Results</h3><p>Successful transmission was observed in 93.33% of <i>C. quadricarinatus</i>, with both adult worms and egg capsules detected, whereas no transmission was observed in <i>M. rosenbergii.</i> THC was significantly higher in experimentally infested <i>C. quadricarinatus</i> (TCq) than in non-infested <i>C. quadricarinatus</i> (NCq) (<i>p</i><0.05). DHC and glucose concentrations differed among host populations. Total protein concentration also differed significantly among host populations, with NMr exhibiting higher concentrations than the other groups, whereas no significant difference was detected between TCq and NCq.</p><h3>Conclusion</h3><p>The results demonstrate successful infestation of <i>C. quadricarinatus</i> but not in <i>M. rosenbergii</i> under the experimental conditions, suggesting differences in host compatibility. Increased THC in experimentally infested <i>C. quadricarinatus</i> indicates a cellular response to symbiont exposure, although experimental stress may also have contributed. Overall, the findings provide restricted host compatibility and highlight that invasive <i>C. quadricarinatus</i> may serve as a reservoir for <i>D. boschmai</i>, facilitating its persistence and dissemination in freshwater ecosystems.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"71 5","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Host Compatibility of the Temnocephalid Diceratocephala boschmai: Experimental Transmission Between Invasive Cherax quadricarinatus and Native Macrobrachium rosenbergii in Malaysia\",\"authors\":\"N. Azri-Shah Norhan, Nor Asma Husna Yusoff, Farizan Abdullah, Ahmad Najmi Ishak, Norainy Mohd Husin, Hin Boo Wee, John Brian Jones, Mhd Ikhwanuddin, Siti Azizah Mohd Nor, Marina Hassan\",\"doi\":\"10.1007/s11686-026-01415-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>The introduction of host-associated symbionts by invasive species threatens native aquatic biodiversity through symbiont spillover and host switching. This study investigated the experimental host-specificity and transmission potential of <i>Diceratocephala boschmai</i> from invasive <i>Cherax quadricarinatus</i> to the native giant freshwater prawn <i>Macrobrachium rosenbergii</i>.</p><h3>Methods</h3><p>Briefly, temnocephalid-infested <i>C. quadricarinatus</i> were exposed to symbiont-free <i>M. rosenbergii</i> and <i>C. quadricarinatus</i> for 30 days. Thereafter, symbiont transmission was assessed, and host hemolymph parameters, including total hemocyte count (THC), differential hemocyte count (DHC), glucose, and protein concentration, were evaluated.</p><h3>Results</h3><p>Successful transmission was observed in 93.33% of <i>C. quadricarinatus</i>, with both adult worms and egg capsules detected, whereas no transmission was observed in <i>M. rosenbergii.</i> THC was significantly higher in experimentally infested <i>C. quadricarinatus</i> (TCq) than in non-infested <i>C. quadricarinatus</i> (NCq) (<i>p</i><0.05). DHC and glucose concentrations differed among host populations. Total protein concentration also differed significantly among host populations, with NMr exhibiting higher concentrations than the other groups, whereas no significant difference was detected between TCq and NCq.</p><h3>Conclusion</h3><p>The results demonstrate successful infestation of <i>C. quadricarinatus</i> but not in <i>M. rosenbergii</i> under the experimental conditions, suggesting differences in host compatibility. Increased THC in experimentally infested <i>C. quadricarinatus</i> indicates a cellular response to symbiont exposure, although experimental stress may also have contributed. 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Host Compatibility of the Temnocephalid Diceratocephala boschmai: Experimental Transmission Between Invasive Cherax quadricarinatus and Native Macrobrachium rosenbergii in Malaysia
Background
The introduction of host-associated symbionts by invasive species threatens native aquatic biodiversity through symbiont spillover and host switching. This study investigated the experimental host-specificity and transmission potential of Diceratocephala boschmai from invasive Cherax quadricarinatus to the native giant freshwater prawn Macrobrachium rosenbergii.
Methods
Briefly, temnocephalid-infested C. quadricarinatus were exposed to symbiont-free M. rosenbergii and C. quadricarinatus for 30 days. Thereafter, symbiont transmission was assessed, and host hemolymph parameters, including total hemocyte count (THC), differential hemocyte count (DHC), glucose, and protein concentration, were evaluated.
Results
Successful transmission was observed in 93.33% of C. quadricarinatus, with both adult worms and egg capsules detected, whereas no transmission was observed in M. rosenbergii. THC was significantly higher in experimentally infested C. quadricarinatus (TCq) than in non-infested C. quadricarinatus (NCq) (p<0.05). DHC and glucose concentrations differed among host populations. Total protein concentration also differed significantly among host populations, with NMr exhibiting higher concentrations than the other groups, whereas no significant difference was detected between TCq and NCq.
Conclusion
The results demonstrate successful infestation of C. quadricarinatus but not in M. rosenbergii under the experimental conditions, suggesting differences in host compatibility. Increased THC in experimentally infested C. quadricarinatus indicates a cellular response to symbiont exposure, although experimental stress may also have contributed. Overall, the findings provide restricted host compatibility and highlight that invasive C. quadricarinatus may serve as a reservoir for D. boschmai, facilitating its persistence and dissemination in freshwater ecosystems.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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