{"title":"SARS-CoV-2 S1-RBD蛋白在Na+/Ca2+饱和蒙脱石上的吸附行为","authors":"Min Xu, Shiqi Li, Qian Chen, Xinhao Wang, Mingqiao Chen, Haoming Wang, Cheng Gu, Xinda Wu","doi":"10.1007/s00128-026-04340-w","DOIUrl":null,"url":null,"abstract":"<p><p>SARS-CoV-2, known for its high transmissibility, spreads via aerosols, enabling long-term environmental persistence. Mineral particles, a key aerosol component, may interact with the virus, yet research remains limited. This study investigated the adsorption of SARS-CoV-2's receptor-binding domain (RBD) protein onto Na<sup>+</sup>- and Ca<sup>2+</sup>-montmorillonite (NaM, CaM) at pH 5 and 9 and ionic strengths of 1 mM and 10 mM. Results showed NaM adsorbed RBD faster and more efficiently than CaM. Higher ionic strength delayed adsorption equilibrium. Adsorption was lower at pH 5 than pH 9, suggesting contributions beyond electrostatic forces (e.g., hydrophobic interactions, hydrogen bonding). Theoretical calculations confirmed experiments and revealed Ca<sup>2+</sup>-mediated bridging between RBD and CaM. This work clarifies viral adsorption mechanisms on clay minerals, aiding environmental risk assessment of virus-mineral interactions.</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"117 4","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Adsorption Behavior of SARS-CoV-2 S1-RBD Protein on Na<sup>+</sup>/Ca<sup>2+</sup>-Saturated Montmorillonites.\",\"authors\":\"Min Xu, Shiqi Li, Qian Chen, Xinhao Wang, Mingqiao Chen, Haoming Wang, Cheng Gu, Xinda Wu\",\"doi\":\"10.1007/s00128-026-04340-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>SARS-CoV-2, known for its high transmissibility, spreads via aerosols, enabling long-term environmental persistence. Mineral particles, a key aerosol component, may interact with the virus, yet research remains limited. This study investigated the adsorption of SARS-CoV-2's receptor-binding domain (RBD) protein onto Na<sup>+</sup>- and Ca<sup>2+</sup>-montmorillonite (NaM, CaM) at pH 5 and 9 and ionic strengths of 1 mM and 10 mM. Results showed NaM adsorbed RBD faster and more efficiently than CaM. Higher ionic strength delayed adsorption equilibrium. Adsorption was lower at pH 5 than pH 9, suggesting contributions beyond electrostatic forces (e.g., hydrophobic interactions, hydrogen bonding). Theoretical calculations confirmed experiments and revealed Ca<sup>2+</sup>-mediated bridging between RBD and CaM. This work clarifies viral adsorption mechanisms on clay minerals, aiding environmental risk assessment of virus-mineral interactions.</p>\",\"PeriodicalId\":501,\"journal\":{\"name\":\"Bulletin of Environmental Contamination and Toxicology\",\"volume\":\"117 4\",\"pages\":\"\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2026-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Environmental Contamination and Toxicology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1007/s00128-026-04340-w\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Environmental Contamination and Toxicology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s00128-026-04340-w","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Adsorption Behavior of SARS-CoV-2 S1-RBD Protein on Na+/Ca2+-Saturated Montmorillonites.
SARS-CoV-2, known for its high transmissibility, spreads via aerosols, enabling long-term environmental persistence. Mineral particles, a key aerosol component, may interact with the virus, yet research remains limited. This study investigated the adsorption of SARS-CoV-2's receptor-binding domain (RBD) protein onto Na+- and Ca2+-montmorillonite (NaM, CaM) at pH 5 and 9 and ionic strengths of 1 mM and 10 mM. Results showed NaM adsorbed RBD faster and more efficiently than CaM. Higher ionic strength delayed adsorption equilibrium. Adsorption was lower at pH 5 than pH 9, suggesting contributions beyond electrostatic forces (e.g., hydrophobic interactions, hydrogen bonding). Theoretical calculations confirmed experiments and revealed Ca2+-mediated bridging between RBD and CaM. This work clarifies viral adsorption mechanisms on clay minerals, aiding environmental risk assessment of virus-mineral interactions.
期刊介绍：
The Bulletin of Environmental Contamination and Toxicology(BECT) is a peer-reviewed journal that offers rapid review and publication. Accepted submissions will be presented as clear, concise reports of current research for a readership concerned with environmental contamination and toxicology. Scientific quality and clarity are paramount.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
