脑内4-羟基他莫昔芬对小鼠脑浸润性B细胞空间限制性creert2介导重组的研究

IF 1.4 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2026-09-21 DOI:10.1016/j.xpro.2026.104856

Mika Schneider, Georgios Perdikaris, Tobias Derfuss, Nicholas S R Sanderson

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引用次数: 0

摘要

依赖于他莫昔芬的重组酶介导技术能够精确控制基因表达的时间，但并不总是提供足够的解剖控制。在这里，我们提出了一种通过在骨蜡中局部注射4-羟基他莫昔芬来限制基因表达的方案。作为一个例子，我们描述了病毒蛋白LMP1和荧光蛋白tdTomato仅在脑浸润性B细胞中的诱导。我们描述了小鼠脑内病毒注射和4-羟基他莫昔芬给药的步骤。有关该协议的使用和执行的完整细节，请参阅Kim等人1。

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Protocol for spatially restricted CreERT2-mediated recombination in mouse brain-infiltrating B cells using intracerebral 4-hydroxy-tamoxifen.

Tamoxifen-dependent, cre-recombinase-mediated techniques enable precise temporal control of gene expression, but do not always provide sufficient anatomical control. Here, we present a protocol to restrict gene expression using local injection of 4-hydroxy-tamoxifen in bone wax. As an example, we describe the induction of the viral protein LMP1 and the fluorescent protein tdTomato only in brain-infiltrating B cells. We describe steps for intracerebral virus injection and 4-hydroxy-tamoxifen administration in mice. For complete details on the use and execution of this protocol, please refer to Kim et al.¹.

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