高通量DNA荧光测序中结构寡核苷酸簇的形成

IF 1.5 4区化学 Q4 CHEMISTRY, ANALYTICAL

Journal of Analytical Chemistry Pub Date : 2026-08-02 DOI:10.1134/S1061934826700607

A. L. Bulyanitsa, D. A. Belov, I. V. Zarutsky, V. V. Manoilov, Ya. I. Alekseev, A. A. Evstrapov

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引用次数: 0

摘要

考虑到各种整体质量指标，本文提出了在填充高通量DNA测序的流动池中形成DNA片段结构簇的相应方案的解决方案。研究了实现多克隆集群检测可能性的条件。根据以下特征的相对重要性，给出了选择聚类形成算法的建议：最小化微孔数量，最小化“丢失”DNA片段和/或使用的微孔的比例，最大化分析速度，以及指定要求的各种组合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Formation of Structured Oligonucleotide Clusters in High-Throughput DNA Sequencing with Fluorescence Detection

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Formation of Structured Oligonucleotide Clusters in High-Throughput DNA Sequencing with Fluorescence Detection

The article presents solutions corresponding to protocols for the formation of structured clusters of DNA fragments in filling a flow cell for the high-throughput DNA sequencing taking into account various integral quality indicators. Conditions for implementing a possibility of the detection of a polyclonal cluster were investigated. Recommendations are given for choosing an algorithm of cluster formation depending on the relative importance of the following characteristics: minimization of the number of microwells, minimization of the fraction of “lost” DNA fragments and/or used microwells, maximization of the speed of analysis, and various combinations of the specified requirements.

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来源期刊

Journal of Analytical Chemistry 化学-分析化学

CiteScore

2.10

自引率

9.10%

发文量

146

审稿时长

13 months

期刊介绍： The Journal of Analytical Chemistry is an international peer reviewed journal that covers theoretical and applied aspects of analytical chemistry; it informs the reader about new achievements in analytical methods, instruments and reagents. Ample space is devoted to problems arising in the analysis of vital media such as water and air. Consideration is given to the detection and determination of metal ions, anions, and various organic substances. The journal welcomes manuscripts from all countries in the English or Russian language.