Patrícia Maria Monteiro, Gabriela Leite Pedroso, Maria Cecília Gorita Dos Santos, Thaísa Maria Vellasco Queiroz Pimenta, Sara Gollino, Mirian Aiko Nakane Matsumoto, Maria Bernadete Sasso Stuani
{"title":"动物模拟肥胖对正畸牙齿移动过程中RANK/RANKL/OPG通路的影响","authors":"Patrícia Maria Monteiro, Gabriela Leite Pedroso, Maria Cecília Gorita Dos Santos, Thaísa Maria Vellasco Queiroz Pimenta, Sara Gollino, Mirian Aiko Nakane Matsumoto, Maria Bernadete Sasso Stuani","doi":"10.1111/ocr.70157","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the impact of simulated obesity in rats on Receptor Activator of Nuclear Factor κB (RANK), Receptor Activator of Nuclear Factor κB Ligand (RANKL) and Osteoprotegerin (OPG) expression during orthodontic tooth movement (OTM).</p><p><strong>Methods: </strong>Forty male Wistar rats 6 weeks old were allocated into non-obese and obese groups. Obesity was simulated by a high-fat diet for 8 weeks. After, the left maxillary first molar was moved by stretched orthodontic nickel-titanium coiled springs. The animals were euthanized after 7 and 14 days of OTM, and the alveolar bone surrounding the roots of the left and right maxillary first molar was dissected to gene expression by qRT-PCR. TaqMan assays for RANK, RANKL, OPG and GAPDH were used, and the values of gene expression were compared by one- and two-way ANOVA with Tukey post-test (alpha = 5%).</p><p><strong>Results: </strong>RANK expression in OTM side at 7 days was statistically different between non- and obese rats (p < 0.01), but not at 14 days (p > 0.05). The RANKL and OPG expression levels on the OTM side did not differ between the groups after 7 or 14 days of OTM.</p><p><strong>Conclusion: </strong>The RANKL and OPG gene expression is not affected by OTM in simulated obese rats. The impact of obesity on RANK gene expression needs to be further studied.</p>","PeriodicalId":19652,"journal":{"name":"Orthodontics & Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impact of Simulated Obesity in Animals on RANK/RANKL/OPG Pathway During Orthodontic Tooth Movement.\",\"authors\":\"Patrícia Maria Monteiro, Gabriela Leite Pedroso, Maria Cecília Gorita Dos Santos, Thaísa Maria Vellasco Queiroz Pimenta, Sara Gollino, Mirian Aiko Nakane Matsumoto, Maria Bernadete Sasso Stuani\",\"doi\":\"10.1111/ocr.70157\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study aimed to evaluate the impact of simulated obesity in rats on Receptor Activator of Nuclear Factor κB (RANK), Receptor Activator of Nuclear Factor κB Ligand (RANKL) and Osteoprotegerin (OPG) expression during orthodontic tooth movement (OTM).</p><p><strong>Methods: </strong>Forty male Wistar rats 6 weeks old were allocated into non-obese and obese groups. Obesity was simulated by a high-fat diet for 8 weeks. After, the left maxillary first molar was moved by stretched orthodontic nickel-titanium coiled springs. The animals were euthanized after 7 and 14 days of OTM, and the alveolar bone surrounding the roots of the left and right maxillary first molar was dissected to gene expression by qRT-PCR. TaqMan assays for RANK, RANKL, OPG and GAPDH were used, and the values of gene expression were compared by one- and two-way ANOVA with Tukey post-test (alpha = 5%).</p><p><strong>Results: </strong>RANK expression in OTM side at 7 days was statistically different between non- and obese rats (p < 0.01), but not at 14 days (p > 0.05). The RANKL and OPG expression levels on the OTM side did not differ between the groups after 7 or 14 days of OTM.</p><p><strong>Conclusion: </strong>The RANKL and OPG gene expression is not affected by OTM in simulated obese rats. The impact of obesity on RANK gene expression needs to be further studied.</p>\",\"PeriodicalId\":19652,\"journal\":{\"name\":\"Orthodontics & Craniofacial Research\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2026-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Orthodontics & Craniofacial Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/ocr.70157\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Orthodontics & Craniofacial Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/ocr.70157","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Impact of Simulated Obesity in Animals on RANK/RANKL/OPG Pathway During Orthodontic Tooth Movement.
Objective: This study aimed to evaluate the impact of simulated obesity in rats on Receptor Activator of Nuclear Factor κB (RANK), Receptor Activator of Nuclear Factor κB Ligand (RANKL) and Osteoprotegerin (OPG) expression during orthodontic tooth movement (OTM).
Methods: Forty male Wistar rats 6 weeks old were allocated into non-obese and obese groups. Obesity was simulated by a high-fat diet for 8 weeks. After, the left maxillary first molar was moved by stretched orthodontic nickel-titanium coiled springs. The animals were euthanized after 7 and 14 days of OTM, and the alveolar bone surrounding the roots of the left and right maxillary first molar was dissected to gene expression by qRT-PCR. TaqMan assays for RANK, RANKL, OPG and GAPDH were used, and the values of gene expression were compared by one- and two-way ANOVA with Tukey post-test (alpha = 5%).
Results: RANK expression in OTM side at 7 days was statistically different between non- and obese rats (p < 0.01), but not at 14 days (p > 0.05). The RANKL and OPG expression levels on the OTM side did not differ between the groups after 7 or 14 days of OTM.
Conclusion: The RANKL and OPG gene expression is not affected by OTM in simulated obese rats. The impact of obesity on RANK gene expression needs to be further studied.
期刊介绍：
Orthodontics & Craniofacial Research - Genes, Growth and Development is published to serve its readers as an international forum for the presentation and critical discussion of issues pertinent to the advancement of the specialty of orthodontics and the evidence-based knowledge of craniofacial growth and development. This forum is based on scientifically supported information, but also includes minority and conflicting opinions.
The objective of the journal is to facilitate effective communication between the research community and practicing clinicians. Original papers of high scientific quality that report the findings of clinical trials, clinical epidemiology, and novel therapeutic or diagnostic approaches are appropriate submissions. Similarly, we welcome papers in genetics, developmental biology, syndromology, surgery, speech and hearing, and other biomedical disciplines related to clinical orthodontics and normal and abnormal craniofacial growth and development. In addition to original and basic research, the journal publishes concise reviews, case reports of substantial value, invited essays, letters, and announcements.
The journal is published quarterly. The review of submitted papers will be coordinated by the editor and members of the editorial board. It is policy to review manuscripts within 3 to 4 weeks of receipt and to publish within 3 to 6 months of acceptance.
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