Petra A McLeod, Ahmed M H Bshina, Peter L Beach, Julian A Guttman
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Flotillin 1 Regulation of Enteropathogenic Escherichia coli Pedestal Length.
Background: The attaching and effacing pathogens, enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC), remodel the host actin cytoskeleton to form actin-rich pedestals that anchor the bacteria atop intestinal epithelial cells. Although pedestal formation requires clathrin-endocytic proteins, the role of clathrin-independent proteins like flotillin 1 remain unclear.
Methods: Flotillin 1 localization in pedestal formation was analyzed using immunofluorescence microscopy during EPEC and EHEC infections in HeLa cells. This protein's function was then examined using competing peptide interference and small interfering RNA (siRNA)-mediated knockdown approaches.
Results: Flotillin 1 localizes to the membranes of EPEC pedestals. Disruption of flotillin 1 membrane association or depletion of flotillin 1 using siRNA resulted in significantly elongated EPEC pedestals. A similar pattern of flotillin 1 localization is seen at EHEC pedestals.
Conclusions: Our work expands current models of attaching and effacing host-pathogen interactions by highlighting a role for flotillin 1 in controlling pedestal length.
期刊介绍：
Published continuously since 1904, The Journal of Infectious Diseases (JID) is the premier global journal for original research on infectious diseases. The editors welcome Major Articles and Brief Reports describing research results on microbiology, immunology, epidemiology, and related disciplines, on the pathogenesis, diagnosis, and treatment of infectious diseases; on the microbes that cause them; and on disorders of host immune responses. JID is an official publication of the Infectious Diseases Society of America.
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