{"title":"间接血凝试验与毒素中和试验测定小鼠血清白喉抗毒素的比较","authors":"S.C. Maheshwari , Suniti B. Sharma , Subhash Ahuja , S.N. Saxena","doi":"10.1016/S0092-1157(87)80004-5","DOIUrl":null,"url":null,"abstract":"<div><p>Serum samples from 42 groups of mice immunized for different immunization periods wich various doses of Adsorbed Diphtheria-Tetanus Vaccine, Adsorbed Diphtheria-Tetanus and Pertussis Vaccine and a standard diphtheria toxoid were assayed for their diphtheria antitoxin content by indirect haemagglutination (IHA) and by toxin neutralization (TN) tests. A very good correlation of 0.91 was obtained between the results of the two methods. There was no statistically significant difference between the IHA and the TN titres obtained. Adsorption with sheep red cells and treatment of the sera with 2-mercaptoethanol had no effect on the IHA titres. The minimum level of antitoxin detectable by the IHA test was 0.00039 IU ml<sup>-1</sup>. IHA proved to be a sensitive, specific and reproducible method which can be used reliably for the assay of diphtheria antitoxin in mouse sera.</p></div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"15 4","pages":"Pages 315-321"},"PeriodicalIF":0.0000,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0092-1157(87)80004-5","citationCount":"5","resultStr":"{\"title\":\"A comparison of the indirect haemagglutination test with the toxin neutralization test for the estimation of diphtheria antitoxin in mouse sera\",\"authors\":\"S.C. Maheshwari , Suniti B. Sharma , Subhash Ahuja , S.N. Saxena\",\"doi\":\"10.1016/S0092-1157(87)80004-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Serum samples from 42 groups of mice immunized for different immunization periods wich various doses of Adsorbed Diphtheria-Tetanus Vaccine, Adsorbed Diphtheria-Tetanus and Pertussis Vaccine and a standard diphtheria toxoid were assayed for their diphtheria antitoxin content by indirect haemagglutination (IHA) and by toxin neutralization (TN) tests. A very good correlation of 0.91 was obtained between the results of the two methods. There was no statistically significant difference between the IHA and the TN titres obtained. Adsorption with sheep red cells and treatment of the sera with 2-mercaptoethanol had no effect on the IHA titres. The minimum level of antitoxin detectable by the IHA test was 0.00039 IU ml<sup>-1</sup>. IHA proved to be a sensitive, specific and reproducible method which can be used reliably for the assay of diphtheria antitoxin in mouse sera.</p></div>\",\"PeriodicalId\":75991,\"journal\":{\"name\":\"Journal of biological standardization\",\"volume\":\"15 4\",\"pages\":\"Pages 315-321\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0092-1157(87)80004-5\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biological standardization\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0092115787800045\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biological standardization","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0092115787800045","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
摘要
采用间接血凝法(IHA)和毒素中和法(TN)测定了42组小鼠不同免疫期接种不同剂量白喉-破伤风吸附疫苗、白喉-破伤风-百日咳吸附疫苗和标准白喉类毒素的血清白喉抗毒素含量。两种方法的相关系数为0.91。所得的IHA与TN滴度无统计学差异。绵羊红细胞吸附和2-巯基乙醇处理血清对IHA滴度没有影响。IHA试验检测到的抗毒素最低水平为0.00039 IU ml-1。结果表明，该方法灵敏、特异、重复性好，可用于小鼠血清中白喉抗毒素的测定。
A comparison of the indirect haemagglutination test with the toxin neutralization test for the estimation of diphtheria antitoxin in mouse sera
Serum samples from 42 groups of mice immunized for different immunization periods wich various doses of Adsorbed Diphtheria-Tetanus Vaccine, Adsorbed Diphtheria-Tetanus and Pertussis Vaccine and a standard diphtheria toxoid were assayed for their diphtheria antitoxin content by indirect haemagglutination (IHA) and by toxin neutralization (TN) tests. A very good correlation of 0.91 was obtained between the results of the two methods. There was no statistically significant difference between the IHA and the TN titres obtained. Adsorption with sheep red cells and treatment of the sera with 2-mercaptoethanol had no effect on the IHA titres. The minimum level of antitoxin detectable by the IHA test was 0.00039 IU ml-1. IHA proved to be a sensitive, specific and reproducible method which can be used reliably for the assay of diphtheria antitoxin in mouse sera.
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