快速轴向分辨率增强显微镜与结构照明

IF 5.7 1区物理与天体物理 Q1 OPTICS

APL Photonics Pub Date : 2024-12-01 DOI:10.1063/5.0239482

Shihang Luo, Ran Gong, Mingcong Zhang, Tao Xu, Wei Ji, Lusheng Gu

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引用次数: 1

摘要

在这项研究中，我们介绍了快速轴向分辨率增强（FARE）显微镜，它采用倾斜条形照明来实现每个平面三张图像的近各向同性三维分辨率。FARE在55 × 55 × 3 μm3下提供高达1.5 Hz的成像速率，轴向分辨率为~ 270 nm。利用这一技术，我们在三维空间观察了内质网的运动，并发现了氧化应激下线粒体形态转变及其与其他细胞器相互作用的新模式。这些发现突出了FARE显微镜在生物医学应用中的巨大潜力。

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Fast axial resolution enhancement microscopy with structured illumination

In this study, we introduce Fast Axial Resolution Enhancement (FARE) microscopy, which employs tilted strip illumination to achieve a near-isotropic three-dimensional resolution with three images per plane. FARE provides an imaging rate of up to 1.5 Hz for 55 × 55 × 3 μm3 with an axial resolution of ∼270 nm. Using this technique, we observed the movement of the endoplasmic reticulum in three dimensions and discovered novel patterns of mitochondrial morphological transitions and their interactions with other organelles under oxidative stress. These findings highlight the significant potential of FARE microscopy for biomedical applications.

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来源期刊

APL Photonics Physics and Astronomy-Atomic and Molecular Physics, and Optics

CiteScore

10.30

自引率

3.60%

发文量

107

审稿时长

19 weeks

期刊介绍： APL Photonics is the new dedicated home for open access multidisciplinary research from and for the photonics community. The journal publishes fundamental and applied results that significantly advance the knowledge in photonics across physics, chemistry, biology and materials science.