血浆活化盐水通过诱导肿瘤细胞凋亡触发巨噬细胞M1极化

IF 3.2 3区物理与天体物理 Q2 PHYSICS, APPLIED

Plasma Processes and Polymers Pub Date : 2026-01-01 DOI:10.1002/ppap.70132

Kejun Qu, Yifan Zhang, Chan Qiang, Zimu Yu, Xixi Jing, Zewei Wang, Jishen Zhang, Zifeng Wang, Dingxin Liu, Hao Zhang, Xin Yang, Mingzhe Rong

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引用次数: 0

摘要

本文研究了表面空气等离子体活化溶液（PAS）中活性氧（ROS）对膀胱癌细胞系MB49的影响及其在肿瘤相关巨噬细胞（tam）极化状态重塑中的作用。该研究揭示了PAS内血浆产生大量活性氧。体外实验表明，PAS对MB49细胞具有激活时间依赖性和显著的细胞毒作用。transwell共培养模型进一步验证表明，PAS预处理MB49细胞可促进RAW264.7巨噬细胞向抗肿瘤M1表型极化。我们的研究揭示了PAS在直接杀伤肿瘤和免疫调节中的双重作用。

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Plasma‐Activated Saline Triggers Macrophage M1 Polarization by Inducing Tumor Cell Apoptosis

ABSTRACT Here, we investigated the effects of aqueous reactive oxygen species (ROS) in surface air plasma‐activated solution (PAS) on the bladder cancer cell line MB49 and their role in remodeling the polarization status of tumor‐associated macrophages (TAMs). The study reveals substantial ROS production by plasma within PAS. In vitro experiments showed that the PAS exhibited an activation time‐dependent and significant cytotoxic effect on MB49 cells. Further validation using a transwell coculture model demonstrated that pretreatment of MB49 cells with PAS promoted the polarization of RAW264.7 macrophages toward the antitumor M1 phenotype. Our study reveals the dual role of PAS in direct tumor killing and immune regulation.

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来源期刊

Plasma Processes and Polymers 物理-高分子科学

CiteScore

6.60

自引率

11.40%

发文量

150

审稿时长

3 months

期刊介绍： Plasma Processes & Polymers focuses on the interdisciplinary field of low temperature plasma science, covering both experimental and theoretical aspects of fundamental and applied research in materials science, physics, chemistry and engineering in the area of plasma sources and plasma-based treatments.