利用阳离子脂质体共同递送聚sirna和氯虫腈复合物：克服小菜蛾抗药性的新策略（鳞翅目：蛾科）

IF 9.2

Advanced Agrochem Pub Date : 2026-06-01 Epub Date: 2025-09-02 DOI:10.1016/j.aac.2025.08.001

ZhiPing Xu , XinQian Wang , Xusheng Shao , YangYang Yang , Kun Yan Zhu

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引用次数: 0

摘要

小菜蛾（Plutella xylostella）是一种广泛分布的害虫，它对十字花科作物具有破坏性影响，并且对杀虫剂，特别是氯虫腈（CAP）具有快速抗性。本研究旨在通过CAP和靶向细胞色素P450 （CYP）基因的多聚sirna共递送，开发新的策略来管理CAP抗性。利用阳离子脂质体（CTL），我们开发了一种独特的CTL- cap /poly-siRNA-CYP复合物共递送系统，其平均粒径为705 nm，表面电荷为+25 mV，多分散指数（PDI）为0.12，表明了良好的配方质量。饲养生物测定显示，这些复合物在中度耐药（CAP- sj）和高度耐药（CAP- qp）小菜蛾中均能显著抑制CYP基因（CYP6BG1和CYP6BF1v4）的表达，与对照组相比，CAP的功效提高了约30%。这种协同方法是第一次将RNAi和杀虫剂结合起来对抗这种害虫的抗药性。这项开创性的研究不仅引入了一种将rnai介导的基因沉默与化学杀虫剂结合的创新方法，而且显著提高了CAP对耐药小菜蛾种群的有效性。基于ctl的多聚sirna和CAP共递送系统成功靶向细胞色素P450抗性基因，增强了氯虫腈对小菜蛾的药效。•RMS处理沉默了抗cap的小菜蛾关键CYP基因（CYP6BG1, CYP6BF1v4）。•CTL-CAP/poly-siRNA-CYP配合物具有稳定的大小、电荷和有效递送的PDI。•Poly-siRNA复合物使耐药小菜蛾菌株的CAP效率提高了约30%。CYP6BF1v4比CYP6BG1对小菜抗性的贡献更大。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Utilizing cationic liposome to co-deliver poly-siRNA and chlorantraniliprole complexes: A new strategy for overcoming insecticide resistance in Plutella xylostella (Lepidoptera: Plutellidae)

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Utilizing cationic liposome to co-deliver poly-siRNA and chlorantraniliprole complexes: A new strategy for overcoming insecticide resistance in Plutella xylostella (Lepidoptera: Plutellidae)

The diamondback moth (Plutella xylostella) is a widely distributed pest recognized for its damaging effects on cruciferous crops and its rapid development of resistance to insecticides, particularly chlorantraniliprole (CAP). This study aims to develop new strategies for managing CAP resistance through the co-delivery of CAP and poly-siRNA targeting cytochrome P450 (CYP) genes. Utilizing a cationic liposome (CTL), we developed a unique co-delivery system for CTL-CAP/poly-siRNA-CYP complexes, characterized by an average particle size of 705 nm, a surface charge of +25 mV, and an impressive polydispersity index (PDI) of 0.12, demonstrating good formulation quality. Feeding bioassays revealed a remarkable capacity of these complexes to suppress CYP genes (CYP6BG1 and CYP6BF1v4) expression in both the moderately CAP-resistant (CAP-SJ) and highly resistant (CAP-QP) P. xylostella, resulting in an about 30 % enhancement in CAP efficacy compared to the control group. This synergistic approach is among the first to combine RNAi and insecticides to combat resistance in this pest. This groundbreaking research not only introduces an innovative method for integrating RNAi-mediated gene silencing with chemical insecticides, but also significantly improves the effectiveness of CAP against resistant P. xylostella populations.

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来源期刊

Advanced Agrochem

CiteScore

3.50

自引率

0.00%

发文量