要有光：目标荧光标记照亮突触的冷冻fib /ET。

IF 4.3 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Structure Pub Date : 2026-05-07 DOI:10.1016/j.str.2026.04.008

Spencer J Rothfuss, Qiangjun Zhou

引用次数: 0

摘要

聚焦离子束铣削暴露出隐藏的细胞区域，如神经网络中的化学突触，用于高分辨率电子显微镜。在这一期的Structure中，Do等人使用荧光标签对突触进行靶向磨粉和成像，成功地去除了混杂物质，并以高分辨率捕获了突触的超微结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Let there be light: Targeted fluorescent labels illuminate cryo-FIB/ET of synapses.

Focused ion beam milling exposes buried cellular regions, such as chemical synapses within neuronal networks, for high-resolution electron microscopy. In this issue of Structure, Do et al.¹ use fluorescent tags to target milling and imaging to synapses, successfully removing confounding material and capturing synaptic ultrastructure in high resolution.

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来源期刊

Structure 生物-生化与分子生物学

CiteScore

8.90

自引率

1.80%

发文量

155

审稿时长

3-8 weeks

期刊介绍： Structure aims to publish papers of exceptional interest in the field of structural biology. The journal strives to be essential reading for structural biologists, as well as biologists and biochemists that are interested in macromolecular structure and function. Structure strongly encourages the submission of manuscripts that present structural and molecular insights into biological function and mechanism. Other reports that address fundamental questions in structural biology, such as structure-based examinations of protein evolution, folding, and/or design, will also be considered. We will consider the application of any method, experimental or computational, at high or low resolution, to conduct structural investigations, as long as the method is appropriate for the biological, functional, and mechanistic question(s) being addressed. Likewise, reports describing single-molecule analysis of biological mechanisms are welcome. In general, the editors encourage submission of experimental structural studies that are enriched by an analysis of structure-activity relationships and will not consider studies that solely report structural information unless the structure or analysis is of exceptional and broad interest. Studies reporting only homology models, de novo models, or molecular dynamics simulations are also discouraged unless the models are informed by or validated by novel experimental data; rationalization of a large body of existing experimental evidence and making testable predictions based on a model or simulation is often not considered sufficient.