A Glucose-Fueled Metal-Organic Framework@Nanofiber Membrane Enables Self-Activated Chemodynamic-Photodynamic Therapy for Diabetic Infections.

Chemodynamic therapy (CDT) and photodynamic therapy (PDT) mediated by reactive oxygen species hold great potential for wound infection management due to their independence from antibiotic resistance. However, chronic wounds with pH > 8 and inadequate H₂O₂ impair the catalytic efficiency required for CDT, and external light irradiation required for PDT damages normal tissues and hinders wound healing. We herein develop a MOF@nanofiber membrane that enables a precise and efficient combination of controlled self-activated CDT and PDT for diabetic infections. Shuttle-shaped PCN-222 MOF nanoparticles act as photosensitizers and platforms for in situ growth of Au nanoparticles with glucose oxidase-like activity and encapsulation of luminol (Lum). These components are integrated into electrospun nanofibrous membranes composed of polyvinyl alcohol and hyaluronic acid, and crosslinked with Fe²⁺ to obtain LPA@PHM(Fe). Mechanistically, the membrane is degraded by bacteria-secreted hyaluronidase and H₂O₂ in infected wounds, producing ·OH and releasing LPA. Au NPs then lower local glucose and pH, and supplement H₂O₂ to enhance CDT and enable Lum-based chemiluminescence resonance energy transfer-mediated PDT. This synergistic antimicrobial effect is verified in vitro and in diabetic wounds. Applied as a band-aid, LPA@PHM(Fe) shows strong potential for promoting healing of diabetic infected wounds.