光通过辣椒CaHY5‐CaBBX2‐CaACS8模块调控辣椒素类生物合成

IF 8.7 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2026-09-03 Epub Date: 2026-04-20 DOI:10.1111/nph.71193

Youxin Yang, 杨有新, Dan Wu, 吴丹, Jiaxin Liu, 柳佳欣, Ying Luo, 罗英, Feng Yang, 杨凤, Huiling Zhu, 朱慧玲, Feng Wang, 王峰, Ting Yu, 余婷, Xiaobin Wang, 王小斌, Jinyin Chen, 陈金印, Huibin Han, 韩惠宾, Yong Zhou, 周勇

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引用次数: 0

摘要

辣椒素是一类独特的生物碱，赋予辣椒果实辛辣的味道。然而，光如何调节辣椒素的生物合成，在很大程度上仍然是未知的。我们对适应光和暗的辣椒果实进行了代谢分析。结果表明，适应黑暗的辣椒果实的辣椒素含量较低，相应的辣椒素生物合成基因（CBGs）转录下调，表明光在辣椒素生物合成中起着至关重要的作用。此外，沉默光信号通路中的关键转录因子CaHY5基因会降低辣椒素含量，抑制CBGs的表达，而短暂过表达CaHY5则会产生完全相反的结果。CaHY5可以结合到CaBBX2和CaACS8启动子中的G - box基序，从而提高它们的转录水平。激活后的CaBBX2与CaACS8启动子中的T/G - box结合，刺激其表达。CaBBX2或CaACS8沉默导致辣椒素水平下降，而它们的瞬时过表达导致辣椒素含量增加。综上所述，我们的研究结果表明，光激活CaHY5‐CaBBX2‐CaACS8调控模块在辣椒素类生物合成中起着关键作用。这些发现为研究光照对辣椒素生物合成的影响以及激活辣椒素生物合成途径的潜在靶点提供了新的见解。

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Light regulates capsaicinoid biosynthesis via the CaHY5-CaBBX2-CaACS8 module in pepper

Capsaicinoids are a class of unique alkaloids that confer the pungent taste to pepper fruits. However, it remains largely unknown how light regulates the biosynthesis of capsaicinoids.
We conducted a metabolic analysis on light- and dark-adapted pepper fruits. The results showed that dark-adapted pepper fruits had lower capsaicinoid contents and correspondingly downregulated transcription of capsaicinoid biosynthetic genes (CBGs), indicating that light plays a crucial role in capsaicinoid biosynthesis. Furthermore, silencing of CaHY5, a pivotal transcription factor gene in the light signaling pathway, decreased the content of capsaicinoid and suppressed the expression of CBGs, whereas transient overexpression of CaHY5 generated exactly opposite results.
CaHY5 can bind to the G-box motif in the promoters of CaBBX2 and CaACS8, thereby enhancing their transcriptional levels. The activated CaBBX2 then binds to the T/G-box in the CaACS8 promoter to stimulate its expression. CaBBX2 or CaACS8 silencing led to decreased levels of capsaicinoids, while their transient overexpression produced increased capsaicinoid contents. Collectively, our results indicated that the light-activated CaHY5-CaBBX2-CaACS8 regulatory module plays a pivotal role in capsaicinoid biosynthesis.
These findings provide new insights into the influence of light on capsaicinoid biosynthesis and potential targets for activation of this biosynthetic pathway in pepper.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.