{"title":"积雪草叶片植物源性外泌体样纳米囊泡（ca - ptens）抗炎症和预防uvb诱导的光老化的潜力","authors":"Anggraini Barlian, Youngkwan Cho, Yulia Rahmah, Annisa Nurrahmi Az Zahra, Hanifa Azka Maulidisya, Noviana Vanawati, Safira Meidina Nursatya","doi":"10.1080/20565623.2026.2654777","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to isolate plant-derived exosome-like nanovesicles from <i>Centella asiatica</i> leaves (CA-PDENs) and evaluate their anti-inflammatory and anti-photoaging potential.</p><p><strong>Materials & methods: </strong>CA-PDENs were isolated via differential centrifugation and polyethylene glycol (PEG) precipitation, then characterized physically and proteomically Anti-inflammatory effects were assessed in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Anti-Photoaging potential was evaluated in ultraviolet B (UVB)-induced 1BR3 fibroblasts. Antibacterial activity against <i>Cutibacterium acnes</i> was also evaluated.</p><p><strong>Results: </strong>CA-PDENs are homogenous, spherical vesicles containing metabolite biosynthesis enzymes. They showed no cytotoxicity and were efficiently internalized. Functionally, CA-PDENs significantly suppressed nitric oxide production, indicating pronounced early-phase anti-inflammatory activity, while CD80, CD206, and interleukin-6 (IL-6) modulation showed consistent but non-significant trends. In fibroblasts, CA-PDENs exhibited strong antioxidant activity, reduced intracellular reactive oxygen species (ROS), attenuated collagen degradation, and enhanced wound closure. Moderate antibacterial activity was also observed.</p><p><strong>Conclusions: </strong>CA-PDENs possess robust anti-inflammatory and photoprotective properties, supporting their potential as multifunctional nanocosmetic ingredients.</p>","PeriodicalId":12568,"journal":{"name":"Future Science OA","volume":"12 1","pages":"2654777"},"PeriodicalIF":2.5000,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078216/pdf/","citationCount":"0","resultStr":"{\"title\":\"The potential of plant-derived exosome-like nanovesicles from <i>Centella asiatica</i> leaves (CA-PDENs) for anti-inflammation and prevention of UVB-induced photoaging.\",\"authors\":\"Anggraini Barlian, Youngkwan Cho, Yulia Rahmah, Annisa Nurrahmi Az Zahra, Hanifa Azka Maulidisya, Noviana Vanawati, Safira Meidina Nursatya\",\"doi\":\"10.1080/20565623.2026.2654777\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>This study aimed to isolate plant-derived exosome-like nanovesicles from <i>Centella asiatica</i> leaves (CA-PDENs) and evaluate their anti-inflammatory and anti-photoaging potential.</p><p><strong>Materials & methods: </strong>CA-PDENs were isolated via differential centrifugation and polyethylene glycol (PEG) precipitation, then characterized physically and proteomically Anti-inflammatory effects were assessed in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Anti-Photoaging potential was evaluated in ultraviolet B (UVB)-induced 1BR3 fibroblasts. Antibacterial activity against <i>Cutibacterium acnes</i> was also evaluated.</p><p><strong>Results: </strong>CA-PDENs are homogenous, spherical vesicles containing metabolite biosynthesis enzymes. They showed no cytotoxicity and were efficiently internalized. Functionally, CA-PDENs significantly suppressed nitric oxide production, indicating pronounced early-phase anti-inflammatory activity, while CD80, CD206, and interleukin-6 (IL-6) modulation showed consistent but non-significant trends. In fibroblasts, CA-PDENs exhibited strong antioxidant activity, reduced intracellular reactive oxygen species (ROS), attenuated collagen degradation, and enhanced wound closure. Moderate antibacterial activity was also observed.</p><p><strong>Conclusions: </strong>CA-PDENs possess robust anti-inflammatory and photoprotective properties, supporting their potential as multifunctional nanocosmetic ingredients.</p>\",\"PeriodicalId\":12568,\"journal\":{\"name\":\"Future Science OA\",\"volume\":\"12 1\",\"pages\":\"2654777\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2026-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078216/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Future Science OA\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/20565623.2026.2654777\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2026/4/11 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future Science OA","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/20565623.2026.2654777","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/4/11 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
The potential of plant-derived exosome-like nanovesicles from Centella asiatica leaves (CA-PDENs) for anti-inflammation and prevention of UVB-induced photoaging.
Aims: This study aimed to isolate plant-derived exosome-like nanovesicles from Centella asiatica leaves (CA-PDENs) and evaluate their anti-inflammatory and anti-photoaging potential.
Materials & methods: CA-PDENs were isolated via differential centrifugation and polyethylene glycol (PEG) precipitation, then characterized physically and proteomically Anti-inflammatory effects were assessed in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Anti-Photoaging potential was evaluated in ultraviolet B (UVB)-induced 1BR3 fibroblasts. Antibacterial activity against Cutibacterium acnes was also evaluated.
Results: CA-PDENs are homogenous, spherical vesicles containing metabolite biosynthesis enzymes. They showed no cytotoxicity and were efficiently internalized. Functionally, CA-PDENs significantly suppressed nitric oxide production, indicating pronounced early-phase anti-inflammatory activity, while CD80, CD206, and interleukin-6 (IL-6) modulation showed consistent but non-significant trends. In fibroblasts, CA-PDENs exhibited strong antioxidant activity, reduced intracellular reactive oxygen species (ROS), attenuated collagen degradation, and enhanced wound closure. Moderate antibacterial activity was also observed.
Conclusions: CA-PDENs possess robust anti-inflammatory and photoprotective properties, supporting their potential as multifunctional nanocosmetic ingredients.
期刊介绍：
Future Science OA is an online, open access, peer-reviewed title from the Future Science Group. The journal covers research and discussion related to advances in biotechnology, medicine and health. The journal embraces the importance of publishing all good-quality research with the potential to further the progress of research in these fields. All original research articles will be considered that are within the journal''s scope, and have been conducted with scientific rigour and research integrity. The journal also features review articles, editorials and perspectives, providing readers with a leading source of commentary and analysis. Submissions of the following article types will be considered: -Research articles -Preliminary communications -Short communications -Methodologies -Trial design articles -Trial results (including early-phase and negative studies) -Reviews -Perspectives -Commentaries
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