Microneedling (MN) combined with albumin gel and liquid platelet-rich fibrin mixtures (Alb-PRF) promotes periodontal soft tissue regeneration in SD rats.

We evaluated the efficiency, as a nonsurgical method, of a combination of microneedling (MN) and a blood-derived biomaterial albumin-platelet-rich fibrin (Alb-PRF) in a rat gingival recession (GR) model for periodontal soft tissue augmentation. Rat blood was used to prepare an Alb-PRF mixture, which was used in combination with MN for periodontal soft tissue regeneration therapy. Twenty-two SD rats, 9-10 weeks of age, were randomly selected; eight were used for blood collection and preparation of Alb-PRF mixtures for use as injectable drugs; three, tested for their ability to release growth factors, and observed by scanning electron microscopy, were used to prepare Alb-PRF mixtures; two were used for the preparation of GR models and observed using stereomicroscopy and micro-CT. The remaining nine rats were randomized into two groups. Group I was (MN treatment group and blank control group) and group II was (MN + Alb-PRF treatment group, Alb-PRF treatment group). All the groups were subjected to GR model establishment. Next, the rats, except the blank control group, were administered the corresponding treatment twice at an interval of 2 weeks. Two weeks after the final dose, each rat was euthanized and the maxillary first molar and periodontal tissue from the surgical region were retrieved. The retrieved samples were evaluated usingin-vivomicroscopy, histopathological assessments, and enzyme-linked immunosorbent assay. The MN + Alb-PRF group exhibited effective periodontal soft tissue regeneration, with significant improvements in parameters such as gingival height, gingival thickness, and clinical crown height, along with an increase in the percentage of collagen fiber area. Administration of MN + Alb-PRF injections to promote periodontal soft tissue regeneration is highly promising strategy for Alb-PRF treatment. The MN group showed most favorable outcomes compared with that of the blank group. Using MN alone and MN in combination with Alb-PRF is effective for promoting periodontal soft tissue regeneration.