山奈酚和cbp -37157对人肺泡2型A549细胞线粒体Ca2+通量的改变

IF 2 4区医学 Q3 PHYSIOLOGY

Physiological research Pub Date : 2026-03-13

K-C Wu, C-Y Chen, L-R Shiao, Z-H Yang, C-M Chuang, Y-M Leung

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引用次数: 0

摘要

线粒体通过线粒体Ca2+单转运体（MCU）和线粒体Na+/Ca2+交换器（mitoNCX）对Ca2+进行处理，参与胞质内Ca2+信号的调节。在这项研究中，我们研究了MCU抑制剂（MCU-i4）、MCU激活剂（山奈酚）和mitoNCX抑制剂（cbp -37157）如何影响激动剂触发的人肺泡2型A549细胞的胞浆Ca2+振荡。虽然MCU的抑制没有显著抑制Ca2+振荡，但山奈酚对MCU的激活显著抑制了振荡活动。CGP-37157对线粒体Ca2+外排的抑制也抑制了Ca2+振荡；山奈酚与CGP 37157的抑制作用不存在叠加性。山奈酚和CGP-37157均引起线粒体基质Ca2+水平升高，但两者的作用不具有加性。综上所述，我们的研究结果表明，肺泡型2 A549细胞中的Ca2+振荡是通过刺激Ca2+摄取到线粒体并阻止Ca2+流出来调节的，这两种情况都会导致Ca2+交通和Ca2+在线粒体基质中的积累受到干扰。关键词线粒体Ca2+振荡线粒体Ca2+单转运体线粒体Na+/Ca2+交换器A549细胞

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Alteration of Mitochondrial Ca2+ Fluxes by Kaempferol and CGP-37157 Regulates Ca2+ Oscillations in Human Alveolar Type 2 A549 Cells.

Mitochondria participate in regulating cytosolic Ca2+ signaling by their Ca2+ handling via mitochondrial Ca2+ uniporter (MCU) and mitochondrial Na+/Ca2+ exchanger (mitoNCX). In this study, we examined how agonist-triggered cytosolic Ca2+ oscillations in human alveolar type 2 A549 cells were affected by an MCU inhibitor (MCU-i4), MCU activator (kaempferol) and mitoNCX inhibitor (CGP-37157). Whilst inhibition of MCU did not significantly repress Ca2+ oscillations, MCU activation by kaempferol considerably dampened oscillatory activities. Inhibition of mitochondrial Ca2+ efflux by CGP-37157 also suppressed Ca2+ oscillations; the suppressive effects of kaempferol and CGP 37157 were not additive. Both kaempferol and CGP-37157 caused a rise in mitochondrial matrix Ca2+ level, but their effects were not additive. Taken together, our results suggest Ca2+ oscillations in alveolar type 2 A549 cells were regulated by stimulating Ca2+ uptake into, and preventing Ca2+ efflux from, the mitochondria, with both cases resulting in disturbed Ca2+ traffic and Ca2+ accumulation in the mitochondrial matrix. Key words Mitochondria " Ca2+ oscillations " Mitochondrial Ca2+ uniporter " Mitochondrial Na+/Ca2+ exchanger " A549 cells.

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来源期刊

Physiological research 医学-生理学

CiteScore

4.00

自引率

4.80%

发文量

108

审稿时长

3 months

期刊介绍： Physiological Research is a peer reviewed Open Access journal that publishes articles on normal and pathological physiology, biochemistry, biophysics, and pharmacology. Authors can submit original, previously unpublished research articles, review articles, rapid or short communications. Instructions for Authors - Respect the instructions carefully when submitting your manuscript. Submitted manuscripts or revised manuscripts that do not follow these Instructions will not be included into the peer-review process. The articles are available in full versions as pdf files beginning with volume 40, 1991. The journal publishes the online Ahead of Print /Pre-Press version of the articles that are searchable in Medline and can be cited.