小肠结肠炎耶尔森菌对三氯生暴露的适应性反应

IF 6.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

LWT - Food Science and Technology Pub Date : 2026-03-01 Epub Date: 2026-02-06 DOI:10.1016/j.lwt.2026.119075

Yingying Zhang , Qiaoxi Chen , Yanzheng Li , Xinquan Hu , Kunyao Luo , Yanzhao Zhu , Yan Zhang , Xingyu Zhu , Linfu Zhou , Gang Zhao , Xiaodong Xia

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引用次数: 0

摘要

小肠结肠炎耶尔森菌（Y. enterocolitica）具有显著的食品安全风险，部分原因是其环境应激恢复能力。三氯生（TCS）作为一种消毒剂被广泛应用于食品加工，小肠结肠炎耶氏菌对TCS应激的反应尚不清楚。本研究评估了tcs诱导小肠结肠炎菌的适应性反应，并通过RNA-Seq检测了基因表达的全局变化。亚致死TCS诱导ATCC 23715产生直接耐药和交叉耐药，包括降低对抗生素的敏感性和增强酸耐受性。猪肉汁增强了对TCS的抵抗力。转录组学显示，TCS攻击后，795个差异表达基因主要与4个关键适应有关。转录组学变化表明了一种代谢权衡：包括核糖体途径（如rps， log2FC =−2.5）和II型分泌系统（pulD, log2FC =−3.1）在内的能量消耗过程的下调伴随着外排泵（acrB, log2FC = 2.3）和鞭毛运动（fliC, log2FC = 2.1）的上调。激活脲酶基因（ureC/E/G, log2FC = 2.8 ~ 3.3），通过氨合成来中和酸性。群体感应调节器（lsrB, log2FC = 2.1）增加AI-2运输，促进自动聚集。上调支链脂肪酸合成基因（fabI, log2FC = 1.9）以维持膜刚性而不改变透性。这些发现说明小肠结肠炎耶氏菌通过多种途径的变化来适应TCS应激，为减少亚致死消毒剂暴露和减轻食品加工环境中耐药性的发展提供了指导。

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Adaptive response of Yersinia enterocolitica to triclosan exposure

Yersinia enterocolitica (Y. enterocolitica) poses significant food safety risks partly due to its environmental stress resilience. Triclosan (TCS) was widely used in food processing as an disinfectant, how Y. enterocolitica responds to TCS stress remains unknown. This study evaluated TCS-induced adaptive responses in Y. enterocolitica and examined global changes in gene expression via RNA-Seq. Sublethal TCS induced direct resistance and cross-resistance in ATCC 23715, including reduced susceptibility to antibiotics and enhanced acid tolerance. Pork juice enhanced TCS resistance. Transcriptomics revealed 795 differentially expressed genes after TCS challenge, mainly related to four key adaptations. Transcriptomic changes indicated a metabolic trade-off: downregulation of energy-costly processes including ribosomal pathways (e.g., rps, log₂FC = −2.5) and type II secretion systems (pulD, log₂FC = −3.1) was accompanied by upregulation of efflux pumps (acrB, log₂FC = 2.3) and flagellar motility (fliC, log₂FC = 2.1). Urease genes (ureC/E/G, log₂FC = 2.8-3.3) were activated to neutralize acidity via ammonia synthesis. Quorum sensing regulators (lsrB, log₂FC = 2.1) increased AI-2 transport, promoting autoaggregation. Branched-chain fatty acid synthesis genes (fabI, log₂FC = 1.9) were upregulated to maintain membrane rigidity without permeability changes. These findings illustrate adaptation of Y. enterocolitica through changes in multiple pathways to TCS stress, providing guidance for minimizing sublethal disinfectant exposure and mitigating the development of resistance in food processing environments.

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来源期刊

LWT - Food Science and Technology 工程技术-食品科技

CiteScore

11.80

自引率

6.70%

发文量

1724

审稿时长

65 days

期刊介绍： LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.