新疆屠宰场羊肉囊菌的分子特征。

IF 3.1 Q2 PARASITOLOGY

Food and Waterborne Parasitology Pub Date : 2026-03-01 Epub Date: 2026-01-31 DOI:10.1016/j.fawpar.2026.e00318

Yafei Zhao , Qiong Zhang , Wenxuan Ma , Yongqiang He , Aiyun Zhao , Meng Qi , Hui Dong

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引用次数: 0

摘要

肌囊虫是一种重要的人畜共患原虫寄生虫。本研究采集了新疆7个城市7个屠宰场354只羊的354份组织样本（骨骼肌样本22份、膈肌样本74份、心脏样本171份、食道样本87份）。以18S rRNA基因为靶点进行PCR筛选，以COX 1基因为靶点进行PCR确认。感染率为30.2%（107/354）。7个城市均呈阳性，塔城感染率最高（95.5%,21/22），库车最低（9.2%,9/98）。组织感染率分别为：骨骼肌95.5%（21/22）、膈肌40.5%（30/74）、心脏24.0%（41/171）、食管17.2%（15/87）。从107份阳性标本中鉴定出2种肉囊菌，分别为柔嫩S.（106）和arieticanis(1)。鉴定出5种短柔链球菌基因型和1种山羊链球菌基因型，均与先前发现的分离株聚类密切。这些结果增强了我们对新疆绵羊肌囊虫病流行病学现状的认识。

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Molecular characteristics of Sarcocystis spp. in sheep from slaughterhouse in Xinjiang, China

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Molecular characteristics of Sarcocystis spp. in sheep from slaughterhouse in Xinjiang, China

Sarcocystis is an important zoonotic protozoan parasites. This study collected 354 tissue samples (22 skeletal muscle samples, 74 diaphragm samples, 171 heart samples, and 87 esophagus samples) from 354 sheep across seven slaughterhouses in seven cities of Xinjiang. Screening for Sarcocystis spp. was performed using PCR targeting the 18S rRNA gene, with confirmation PCR targeting the COX 1 gene. The infection rate was determined to be 30.2% (107/354). All seven cities tested positive for Sarcocystis, with the highest infection rate in Tacheng (95.5%, 21/22) and the lowest infection rate in Kuqa (9.2%, 9/98). The infection rates by tissue were as follows: 95.5% (21/22) in skeletal muscles, 40.5% (30/74) in diaphragm, 24.0% (41/171) in heart, and 17.2% (15/87) in esophageal, respectively. Two Sarcocystis species were identified from the 107 positive samples: S. tenella (n = 106) and S. arieticanis (n = 1). Five genotypes of S. tenella and one genotype of S. arieticanis were identified, all of which clustered closely with previously discovered isolates. These results enhance our understanding of the epidemiological status of Sarcocystis in sheep in Xinjiang.

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来源期刊

Food and Waterborne Parasitology Immunology and Microbiology-Parasitology

CiteScore

5.10

自引率

4.00%

发文量

审稿时长

13 weeks

期刊介绍： Food and Waterborne Parasitology publishes high quality papers containing original research findings, investigative reports, and scientific proceedings on parasites which are transmitted to humans via the consumption of food or water. The relevant parasites include protozoa, nematodes, cestodes and trematodes which are transmitted by food or water and capable of infecting humans. Pertinent food includes products of animal or plant origin which are domestic or wild, and consumed by humans. Animals and plants from both terrestrial and aquatic sources are included, as well as studies related to potable and other types of water which serve to harbor, perpetuate or disseminate food and waterborne parasites. Studies dealing with prevalence, transmission, epidemiology, risk assessment and mitigation, including control measures and test methodologies for parasites in food and water are of particular interest. Evidence of the emergence of such parasites and interactions among domestic animals, wildlife and humans are of interest. The impact of parasites on the health and welfare of humans is viewed as very important and within scope of the journal. Manuscripts with scientifically generated information on associations between food and waterborne parasitic diseases and lifestyle, culture and economies are also welcome. Studies involving animal experiments must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences.