Association of Variants of 786T>C (rs2070744) Polymorphism of the NOS3 Gene with Age-Dependent Electrophysiological Disorders in Myocardium.

The aim of the study was to examine associations of ECG signal micro-alternations values with various variants of the 786T>C (rs2070744) polymorphism of the NOS3 gene in cohorts of mature and elderly male Northerners.

Materials and methods: One hundred forty male residents of the Magadan Region (Russia) aged 25-80 were included in the study. All the examinees were classified into mature and elderly. Men of different ages were divided into two groups based on the presence/ absence of the NOS3*C allele in their genotypes: group 1 consisted of homozygous men (TT genotype); group 2 - of heterozygous and homozygous carriers of the NOS3*C allele (TC+CC genotypes). Genetic SNP testing and methods for assessing the characteristics of cardiac variance mapping were used.

Results: The study revealed age-associated impairment of dispersion characteristics of ECG signal, the "Myocardium" and "Rhythm" indices, as well as myocardial electrophysiological disorders, which were more characteristic of the elderly men who were carriers of the minor NOS3*C allele (genotypes TC+CC). This was accompanied by a synchronous increase in the "rigidity" of the matrix of ECG dispersion mapping indicators. The two-factor dispersion analysis showed that the 786T>C (rs2070744) polymorphism of the NOS3 gene significantly influenced the ventricular hypertrophy (G9) at simultaneous cumulative impact with the age factor: the "Age" factor on the "Myocardium" index, right and left ventricular repolarization (G5, G6), and their depolarization symmetry (G7).

Conclusion: The results indicate that monitoring studies of the main characteristics of ECG dispersion mapping parameters in addition to the evaluation of 786T>C (rs2070744) polymorphism of the NOS3 gene must be given emphasis since it is crucial for the formation of personalized and preventive medicine. It has been established that the presence of the minor allele NOS3*C of the polymorphism 786T>C (rs2070744) of the NOS3 locus can be considered as an additional risk factor for age-associated electrophysiological disorders of the myocardium.