{"title":"Komagataella phaffii：生产增值化学品的多功能平台","authors":"Qiang Zhou , Jiahui Yang , Wenming Zhang , Wankui Jiang , Jianxiang Chen , Lei Chen , Yujia Jiang , Fengxue Xin","doi":"10.1016/j.greenca.2025.01.004","DOIUrl":null,"url":null,"abstract":"<div><div>The development of green biomanufacturing technologies centered around advanced microbial cells has emerged as a hot research field in synthetic biology. As known, <em>Komagataella phaffii</em> is an unconventional yeast with unique biological characteristics, which is generally regarded as safe (GRAS). Owing to its wide substrate spectrum, <em>K. phaffii</em> has been widely genetically modified for valuable chemicals such as organic acids, fatty acids and some nature products. Actually, <em>K. phaffii</em> is very well known for the protein expression, and few reviews have systemically addressed the value-added chemicals by <em>K. phaffii</em>. Accordingly, this review will introduce the most advanced development of genetic editing tools of <em>K. phaffii</em>. Recent progresses and bottlenecks in the production of high-value chemicals using <em>K. phaffii</em> will also be summarized. Finally, future perspectives for the utilization of <em>K. phaffii</em> as a chassis cell used for high-value products synthesis will be discussed.</div></div>","PeriodicalId":100595,"journal":{"name":"Green Carbon","volume":"3 4","pages":"Pages 407-418"},"PeriodicalIF":14.2000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Komagataella phaffii: A versatile platform for the production of value-added chemicals\",\"authors\":\"Qiang Zhou , Jiahui Yang , Wenming Zhang , Wankui Jiang , Jianxiang Chen , Lei Chen , Yujia Jiang , Fengxue Xin\",\"doi\":\"10.1016/j.greenca.2025.01.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The development of green biomanufacturing technologies centered around advanced microbial cells has emerged as a hot research field in synthetic biology. As known, <em>Komagataella phaffii</em> is an unconventional yeast with unique biological characteristics, which is generally regarded as safe (GRAS). Owing to its wide substrate spectrum, <em>K. phaffii</em> has been widely genetically modified for valuable chemicals such as organic acids, fatty acids and some nature products. Actually, <em>K. phaffii</em> is very well known for the protein expression, and few reviews have systemically addressed the value-added chemicals by <em>K. phaffii</em>. Accordingly, this review will introduce the most advanced development of genetic editing tools of <em>K. phaffii</em>. Recent progresses and bottlenecks in the production of high-value chemicals using <em>K. phaffii</em> will also be summarized. Finally, future perspectives for the utilization of <em>K. phaffii</em> as a chassis cell used for high-value products synthesis will be discussed.</div></div>\",\"PeriodicalId\":100595,\"journal\":{\"name\":\"Green Carbon\",\"volume\":\"3 4\",\"pages\":\"Pages 407-418\"},\"PeriodicalIF\":14.2000,\"publicationDate\":\"2025-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Green Carbon\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2950155525000126\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/3/12 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Green Carbon","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950155525000126","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/12 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Komagataella phaffii: A versatile platform for the production of value-added chemicals
The development of green biomanufacturing technologies centered around advanced microbial cells has emerged as a hot research field in synthetic biology. As known, Komagataella phaffii is an unconventional yeast with unique biological characteristics, which is generally regarded as safe (GRAS). Owing to its wide substrate spectrum, K. phaffii has been widely genetically modified for valuable chemicals such as organic acids, fatty acids and some nature products. Actually, K. phaffii is very well known for the protein expression, and few reviews have systemically addressed the value-added chemicals by K. phaffii. Accordingly, this review will introduce the most advanced development of genetic editing tools of K. phaffii. Recent progresses and bottlenecks in the production of high-value chemicals using K. phaffii will also be summarized. Finally, future perspectives for the utilization of K. phaffii as a chassis cell used for high-value products synthesis will be discussed.
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