{"title":"用于共递送香芹酚和百里香酚的细菌纳米纤维素膜：理化性质和体外研究。","authors":"Gabbai-Armelin Paulo Roberto, De Oliveira Analú Barros, Rodero Camila Fernanda, Silva Jhonatan Miguel, Fernandes Natalie Aparecida Rodrigues, Paiva Robert, Dametto Alessandra Cristina, Chorilli Marlus, Barud Hernane da Silva, Brighenti Fernanda Lourenção","doi":"10.2174/0115680266377999250929122859","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Skin wounds represent a worldwide problem. Biopolymers have been attracting interest in healthcare products for wound dressing. Among these, bacterial nanocellulose membranes (BNC) are attractive for their unique structure, but they lack antimicrobial activity. Thus, the incorporation of the monoterpenes Carvacrol (Car) and Thymol (Thy) - which present antimicrobial and healing properties - toward the improvement of skin wound healing, consists of an appealing approach. This research aimed to produce and characterize nanocellulose membranes containing carvacrol and/or thymol, and investigate their release behavior, cytotoxicity, and antimicrobial properties.</p><p><strong>Method: </strong>BNC/Car, BNC/Thy, and BNC/Car-Thy membranes were produced at doses of 0.1 and 1.0 mg/cm2.</p><p><strong>Results: </strong>The natural components incorporation into the nanocellulose did not interfere with the ultra-structure or its physical characteristics. Pilot studies showed that membranes with 1.0 mg/cm² of monoterpenes were toxic to fibroblasts. Therefore, all further studies used the lower dose of 0.1 mg/cm². Release experiments showed a burst release between 2-4 h with sustained release till 24 h, reaching around 80% of the initial amount of the incorporated monoterpenes. Studies with fibroblast and keratinocytes indicated no cytotoxicity and that cells could proliferate over the BNC/Car- Thy membranes. Microbiological studies suggested some antimicrobial potential of the BNC doped with Car and Thy.</p><p><strong>Discussion: </strong>BNC membranes incorporated with Car and Thy were successfully produced and the monoterpenes incorporation into the BNC did not interfere with either ultra-structure or with its physico- chemical characteristics. Natural products incorporation induced cell proliferation and presented antimicrobial properties, besides increasing the solubility and stability of these natural compounds.</p><p><strong>Conclusion: </strong>This innovative biomaterial has the potential for healthcare products.</p>","PeriodicalId":11076,"journal":{"name":"Current topics in medicinal chemistry","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bacterial Nanocellulose Membranes for Codelivery of Carvacrol and Thymol: Physico-chemical Characterization and In vitro Studies.\",\"authors\":\"Gabbai-Armelin Paulo Roberto, De Oliveira Analú Barros, Rodero Camila Fernanda, Silva Jhonatan Miguel, Fernandes Natalie Aparecida Rodrigues, Paiva Robert, Dametto Alessandra Cristina, Chorilli Marlus, Barud Hernane da Silva, Brighenti Fernanda Lourenção\",\"doi\":\"10.2174/0115680266377999250929122859\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Skin wounds represent a worldwide problem. Biopolymers have been attracting interest in healthcare products for wound dressing. Among these, bacterial nanocellulose membranes (BNC) are attractive for their unique structure, but they lack antimicrobial activity. Thus, the incorporation of the monoterpenes Carvacrol (Car) and Thymol (Thy) - which present antimicrobial and healing properties - toward the improvement of skin wound healing, consists of an appealing approach. This research aimed to produce and characterize nanocellulose membranes containing carvacrol and/or thymol, and investigate their release behavior, cytotoxicity, and antimicrobial properties.</p><p><strong>Method: </strong>BNC/Car, BNC/Thy, and BNC/Car-Thy membranes were produced at doses of 0.1 and 1.0 mg/cm2.</p><p><strong>Results: </strong>The natural components incorporation into the nanocellulose did not interfere with the ultra-structure or its physical characteristics. Pilot studies showed that membranes with 1.0 mg/cm² of monoterpenes were toxic to fibroblasts. Therefore, all further studies used the lower dose of 0.1 mg/cm². Release experiments showed a burst release between 2-4 h with sustained release till 24 h, reaching around 80% of the initial amount of the incorporated monoterpenes. Studies with fibroblast and keratinocytes indicated no cytotoxicity and that cells could proliferate over the BNC/Car- Thy membranes. Microbiological studies suggested some antimicrobial potential of the BNC doped with Car and Thy.</p><p><strong>Discussion: </strong>BNC membranes incorporated with Car and Thy were successfully produced and the monoterpenes incorporation into the BNC did not interfere with either ultra-structure or with its physico- chemical characteristics. Natural products incorporation induced cell proliferation and presented antimicrobial properties, besides increasing the solubility and stability of these natural compounds.</p><p><strong>Conclusion: </strong>This innovative biomaterial has the potential for healthcare products.</p>\",\"PeriodicalId\":11076,\"journal\":{\"name\":\"Current topics in medicinal chemistry\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2025-10-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current topics in medicinal chemistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2174/0115680266377999250929122859\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current topics in medicinal chemistry","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2174/0115680266377999250929122859","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Bacterial Nanocellulose Membranes for Codelivery of Carvacrol and Thymol: Physico-chemical Characterization and In vitro Studies.
Introduction: Skin wounds represent a worldwide problem. Biopolymers have been attracting interest in healthcare products for wound dressing. Among these, bacterial nanocellulose membranes (BNC) are attractive for their unique structure, but they lack antimicrobial activity. Thus, the incorporation of the monoterpenes Carvacrol (Car) and Thymol (Thy) - which present antimicrobial and healing properties - toward the improvement of skin wound healing, consists of an appealing approach. This research aimed to produce and characterize nanocellulose membranes containing carvacrol and/or thymol, and investigate their release behavior, cytotoxicity, and antimicrobial properties.
Method: BNC/Car, BNC/Thy, and BNC/Car-Thy membranes were produced at doses of 0.1 and 1.0 mg/cm2.
Results: The natural components incorporation into the nanocellulose did not interfere with the ultra-structure or its physical characteristics. Pilot studies showed that membranes with 1.0 mg/cm² of monoterpenes were toxic to fibroblasts. Therefore, all further studies used the lower dose of 0.1 mg/cm². Release experiments showed a burst release between 2-4 h with sustained release till 24 h, reaching around 80% of the initial amount of the incorporated monoterpenes. Studies with fibroblast and keratinocytes indicated no cytotoxicity and that cells could proliferate over the BNC/Car- Thy membranes. Microbiological studies suggested some antimicrobial potential of the BNC doped with Car and Thy.
Discussion: BNC membranes incorporated with Car and Thy were successfully produced and the monoterpenes incorporation into the BNC did not interfere with either ultra-structure or with its physico- chemical characteristics. Natural products incorporation induced cell proliferation and presented antimicrobial properties, besides increasing the solubility and stability of these natural compounds.
Conclusion: This innovative biomaterial has the potential for healthcare products.
期刊介绍：
Current Topics in Medicinal Chemistry is a forum for the review of areas of keen and topical interest to medicinal chemists and others in the allied disciplines. Each issue is solely devoted to a specific topic, containing six to nine reviews, which provide the reader a comprehensive survey of that area. A Guest Editor who is an expert in the topic under review, will assemble each issue. The scope of Current Topics in Medicinal Chemistry will cover all areas of medicinal chemistry, including current developments in rational drug design, synthetic chemistry, bioorganic chemistry, high-throughput screening, combinatorial chemistry, compound diversity measurements, drug absorption, drug distribution, metabolism, new and emerging drug targets, natural products, pharmacogenomics, and structure-activity relationships. Medicinal chemistry is a rapidly maturing discipline. The study of how structure and function are related is absolutely essential to understanding the molecular basis of life. Current Topics in Medicinal Chemistry aims to contribute to the growth of scientific knowledge and insight, and facilitate the discovery and development of new therapeutic agents to treat debilitating human disorders. The journal is essential for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important advances.
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