克氏原螯虾（Procambarus clarkii）的全身葡萄糖摄取：通过[18F]FDG MicroPET成像研究性别二态性。

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-10-18 DOI:10.1016/j.etap.2025.104849

Arturo Avendaño-Estrada , Miguel Angel Avila-Rodríguez , Jesús Hernández-Falcón , Karina Mendoza-Ángeles

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引用次数: 0

摘要

小龙虾因其适应性和抗性而被广泛应用于生物医学研究。虽然遗传学、分子生物学、行为评估、电生理学和显微镜技术已被用于研究这些甲壳类动物，但使用成像技术进行体内代谢评估仍然很少。从这个角度来看，在甲壳类动物模型中使用微正电子发射断层扫描（MicroPET）成像代表了一种了解这些生物代谢过程和评估潜在环境对水生物种影响的新方法。目的：利用MicroPET成像技术评估雄性和雌性小龙虾对[18F]FDG的局部摄取，并寻找最佳扫描采集时间。方法：给药[18F]FDG（7.4±1.2 MBq）后，对成年雄性和雌性小龙虾（n=10只/性，30-40g）进行1小时MicroPET扫描（6帧/ 10min）。计算脑、性腺、绿腺、心脏和腹侧神经索神经节的标准化摄取值（SUV）。结果：脑、绿腺和食道下神经节的代谢活性最高。[18F]FDG摄取与性别相关的显著差异仅在性腺中观察到，雌性的摄取高于雄性。其他结构间无明显差异；雄螯虾的变异系数（44.33 ~ 92.7%）高于雌螯虾（13.11 ~ 46.55%）。探索性结构间相关分析显示，两性沿腹侧神经索均存在高耦合，雄性的心/绿腺和腹部神经节之间的协调性更强（Δρ高达~0.8），提示性别依赖性代谢整合。结论：微pet成像是一种有价值的工具，可以在10分钟的单次扫描（给药后40分钟）内评估小龙虾的代谢活性和性别二态性。这些发现为进一步研究这些差异的生理生化基础奠定了基础。

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Whole-body glucose uptake in crayfish (Procambarus clarkii): A study of sexual dimorphism via [18F]FDG MicroPET imaging

Crayfish has been used in biomedical research due to their adaptability and resistance. While genetics, molecular biology, behavior assessments, electrophysiology, and microscopy techniques have been employed to study these crustaceans, in vivo metabolic evaluations using imaging techniques remain scarce. From this perspective, the use of micro positron emission tomography (MicroPET) imaging in crustacean models represents a novel approach to understand metabolic processes in these organisms and evaluating potential environmental impacts on aquatic species.

Objective

To assess the regional uptake of [¹⁸F]FDG in male and female crayfish using MicroPET imaging and to find the optimal scan acquisition time.

Methods

Adult male and female crayfish (n = 10/sex, 30–40 g) underwent 1-hour MicroPET scans (6 frames of 10 min) after administration of [¹⁸F]FDG (7.4 ± 1.2 MBq). Standardized uptake values (SUV) were calculated for the brain, gonads, green gland, heart, and ganglia of ventral nerve cord.

Results

The brain, green gland, and subesophageal ganglion exhibited the highest metabolic activity. Significant differences in [¹⁸F]FDG uptake related to sex were observed only in the gonads, with females showing higher uptake than males. No significant differences were found in other structures; nevertheless, male crayfish showed a higher coefficient of variation (44.33–92.7 %) than females (13.11–46.55 %). Exploratory inter-structure correlation analysis showed uniformly high coupling along the ventral nerve cord in both sexes, with males exhibiting stronger coordination between the heart/green gland and abdominal ganglia (Δρ up to ∼0.8), suggesting sex-dependent metabolic integration.

Conclusions

MicroPET imaging is a valuable tool for assessing metabolic activity and sexual dimorphism in crayfish in a single 10-minute scan (40 min after dose administration). These findings provide a groundwork for further studies investigating the physiological and biochemical basis of these differences.

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.