利用涡虫迁移率评价苯扎氯铵的毒理学：人工和数字跟踪方法的比较。

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-10-20 DOI:10.1016/j.etap.2025.104850

Manel M. Habel , Adrian C. Williams , Vitaliy V. Khutoryanskiy

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引用次数: 0

摘要

3rs原则——减少、改进和替代——鼓励最大限度地减少动物使用，改进实验设计，开发毒理学测试的替代模型。在这些模型中，涡虫（水生扁虫）由于其简单的解剖结构、高度的环境敏感性、卓越的再生能力和易于实验室维护而在药理学、再生医学和毒理学方面受到越来越多的关注。在这项研究中，我们使用人工评估和Lolitrack视频跟踪软件检测了苯扎氯铵（BAC）对地中海Schmidtea的运动活性的影响。苯扎氯铵是一种常用的具有抗菌和增强渗透性的药物赋形剂，也是一种已知的环境毒物。检测6种浓度BAC （5 ~ 1000μg/mL）和阴性对照。两种方法都显示，随着时间的推移，运动活动总体上有所减少，尽管人工分析表明，在较低浓度下有短暂的刺激。基于软件的方法具有较高的可靠性、精密度和客观性，可用于涡虫毒性评价。

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Toxicological assessment of benzalkonium chloride using planaria mobility: A comparison of manual and digital tracking methods

The principle of the 3Rs—Reduction, Refinement, and Replacement—encourages minimizing animal use, improving experimental design, and developing alternative models for toxicology testing. Among such models, planaria (aquatic flatworms) have gained increasing attention in pharmacology, regenerative medicine, and toxicology because of their simple anatomy, high environmental sensitivity, exceptional regenerative ability, and ease of laboratory maintenance. In this study, we examined the effects of benzalkonium chloride (BAC)—a commonly used pharmaceutical excipient with antimicrobial and permeability-enhancing properties, as well as a known environmental toxicant—on the locomotor activity of Schmidtea mediterranea using both manual assessment and Lolitrack video-tracking software. Six concentrations of BAC (5–1000 μg/mL) and a negative control were tested. Both approaches showed an overall reduction in locomotor activity over time, though manual analysis indicated a transient stimulation at lower concentrations. The software-based method demonstrated greater reliability, precision, and objectivity, making it preferable for toxicity evaluation in planaria.

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.