基于聚合物的比例荧光探针的合理设计，用于追踪脂滴的极性变化和异质性

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-10-13 DOI:10.1016/j.snb.2025.138987

Lisha Yang , Xue Sun , Juan Li , Xinying Jing , Xiuqi Kong , Minggang Tian , Faqi Yu , Weiying Lin

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引用次数: 0

摘要

脂滴是细胞内脂质储存的重要细胞器，在生命活动中起着多方面的重要作用。ld极性异常变化可引起代谢性疾病、神经退行性疾病、心血管疾病等健康问题。因此，开发一种探头来监测ld的极性变化是很重要的。本文提出了一种新型的基于聚合物的比例荧光探针NP-PEGMA。该探针NP-PEGMA结合了非常规荧光聚合物和极性响应荧光平台，能够有效监测ld的极性变化，具有良好的选择性和光稳定性。结果表明，NP-PEGMA可以监测细胞在各种生理条件和程序性细胞死亡情况下ld的极性变化，也可以跟踪LPS和缺氧刺激下生物体ld的极性变化。此外，NP-PEGMA还实现了对细胞和小鼠组织中ld极性异质性的追踪。因此，这项工作为追踪lld的极性变化和异质性以及研究lld相关疾病提供了有用的工具。

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Rational design of a polymer-based ratiometric fluorescent probe for tracing the polarity changes and heterogeneity of lipid droplets

Lipid droplets (LDs) are key organelles for intracellular storage of lipids and play a multifaceted and important role in life activities. Abnormal polarity changes of LDs may cause health problems such as metabolic diseases, neurodegenerative diseases, and cardiovascular diseases. Therefore, it is important to develop a probe to monitor the polarity changes of LDs. In this paper, a novel polymer-based ratiometric fluorescent probe NP-PEGMA is proposed. The probe NP-PEGMA, combined unconventional fluorescent polymer with a polarity-responsive fluorescent platform, can effectively monitor the polarity changes of LDs with good selectivity and photostability. The results show that NP-PEGMA can be used to monitor the polarity changes of LDs in cells under various physiological conditions and programmed cell death as well as can also track the polarity changes of LDs in living organisms under LPS and hypoxic stimuli. Moreover, NP-PEGMA achieves tracing the polarity heterogeneity of LDs in cells and mouse tissues. Therefore, this work provides a useful tool for tracing the polarity changes and heterogeneity of LDs and studying LDs-related diseases.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.