酵母提取物作为微生物诱导碳酸盐沉淀铜修复的双刃剑：解开营养配体困境

IF 7.8 2区环境科学与生态学 Q1 ENGINEERING, CHEMICAL

Process Safety and Environmental Protection Pub Date : 2025-10-16 DOI:10.1016/j.psep.2025.108028

Zijia Zhang , Keqiang Zhou , Zijing Lu , J. Viridiana Garcia-Meza , Zhenbin Wu , Ling Xia , Xiheng Hu

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引用次数: 0

摘要

微生物诱导碳酸盐沉淀（MICP）是一种很有前途的重金属修复策略，但有机营养物在调节金属固存中的作用仍未得到充分研究。本研究系统地研究了酵母提取物（YE）在MICP中去除铜（Cu 2 +）的双重功能，重点研究了它作为营养源和竞争配体的相互作用。采用从铜尾矿中分离出的一株高解尿、耐Cu 2 +的菌株xylanilyticus SX1，对YE的浓度依赖性进行了评价。结果表明，YE（0-2.0 g/L）增强了SX1的生长和Cu 2 +的耐受性，而3.0 g/L由于营养不平衡而抑制了SX1的生长。矛盾的是，Cu 2 +的去除效率与YE浓度呈负相关，从62.8 %（1.0 g/L YE）下降到6.8 %（3.0 g/L YE）。矿物学分析（XRD、FT-IR、XPS）表明，在1.0 ~ 2.0 g/L YE条件下，Cu₂（OH）₂CO₃沉淀是主要机理，而在3.0 g/L YE条件下则以吸附为主。竞争配体交换吸附阴极溶出伏安法（CLE-ACSV）和UV-vis光谱证实了Cu 2 + - ye强配位（K 'CuYE = 1.2651 × 10 ¹⁰- 1.78238 × 10 ¹⁰），阻碍了碳酸盐的成矿作用。这些发现强调了微生物活力和Cu 2 +固定效果之间的关键权衡，强调了优化MICP系统中有机营养水平的必要性。

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Yeast extract as a double-edged sword in microbial induced carbonate precipitation for copper remediation: Unraveling the nutrient - ligand dilemma

Microbial-induced carbonate precipitation (MICP) is a promising strategy for heavy metal remediation, yet the role of organic nutrients in modulating metal sequestration remains underexplored. This study systematically investigates the dual functionality of yeast extract (YE) in MICP for copper (Cu²⁺) removal, focusing on its interplay as a nutrient source and a competing ligand. A highly ureolytic and Cu²⁺-tolerant strain, Lysinibacillus xylanilyticus SX1, isolated from copper tailings, was employed to evaluate YE's concentration-dependent effects. Results revealed that YE (0–2.0 g/L) enhanced SX1 growth and Cu²⁺ tolerance, while 3.0 g/L inhibited growth due to nutrient imbalance. Paradoxically, Cu²⁺ removal efficiency inversely correlated with YE concentration, declining from 62.8 % (1.0 g/L YE) to 6.8 % (3.0 g/L YE). Mineralogical analyses (XRD, FT-IR, XPS) identified Cu₂(OH)₂CO₃ precipitation as the dominant mechanism at 1.0–2.0 g/L YE, whereas adsorption prevailed at 3.0 g/L YE. Competitive ligand exchange-adsorptive cathodic stripping voltammetry (CLE-ACSV) and UV–vis spectroscopy confirmed strong Cu²⁺-YE coordination (K′_CuYE = 1.2651 × 10 ¹⁰ - 1.78238 × 10 ¹⁰), which hindered carbonate mineralization. These findings highlight a critical trade-off between microbial viability and Cu²⁺ immobilization efficacy, emphasizing the necessity to optimize organic nutrient levels in MICP systems.

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来源期刊

Process Safety and Environmental Protection 环境科学-工程：化工

CiteScore

11.40

自引率

15.40%

发文量

929

审稿时长

8.0 months

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