{"title":"有振动和无振动的光功能化对钛种植体骨整合的影响：体外比较研究。","authors":"Priyanka Patra, Rajyalakshmi Ravuri, Praveen Mamidi, Gautam Kumar Annojjula, Tejaswi Manasa Emany, Chittaranjan Bogishetty","doi":"10.4103/jips.jips_194_25","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>Enhancing early cell-implant interaction is vital for successful osseointegration. This in vitro study aimed to evaluate the combined effect of photofunctionalization and low-frequency mechanical vibration on osteoblast cells derived from human placental tissue cultured on titanium surfaces.</p><p><strong>Settings and design: </strong>Following approval from the Institutional Ethics Committee and informed consent, a controlled in vitro experimental study was conducted in the Department of Prosthodontics in collaboration with a certified cell culture laboratory.</p><p><strong>Materials and methods: </strong>Osteoblast cells were isolated and cultured. Titanium implant surface was photofunctionalized using ultraviolet C (UV-C) light (254 nm) for 48 h. Cells were seeded onto the implant surface and divided into three groups: Group 1: Control (untreated titanium), Group 2: UV-treated titanium, and Group 3: UV-treated titanium implant with mechanical vibration (20 Hz for 3 min/day for 3 days) to osteoblast cells. Cell proliferation was assessed using MTT assay, mineralization via Alizarin Red S staining, and osteogenic gene expression (runt-related transcription factor-2, Alkaline phosphatase, OCN) through reverse transcription polymerase chain reaction.</p><p><strong>Statistical analysis used: </strong>Data were analyzed using analysis of variance with Tukey's post hoc test (P < 0.05).</p><p><strong>Results: </strong>The combination of photofunctionalization and mechanical vibration (Group 3) significantly enhanced cell proliferation, mineral deposition, and osteogenic gene expression compared to Groups 1 and 2 (P < 0.05). While photofunctionalization alone improved osteoblast activity, the synergistic application of mechanical vibration further amplified the response.</p><p><strong>Conclusion: </strong>The combination of photofunctionalization and low-frequency vibration significantly enhances the osteogenic response of osteoblast cells to titanium, indicating its potential to improve implant osseointegration.</p>","PeriodicalId":22669,"journal":{"name":"The Journal of Indian Prosthodontic Society","volume":"25 4","pages":"334-339"},"PeriodicalIF":1.0000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of photofunctionalization with and without vibration on osseointegration of titanium implants: A comparative in vitro study.\",\"authors\":\"Priyanka Patra, Rajyalakshmi Ravuri, Praveen Mamidi, Gautam Kumar Annojjula, Tejaswi Manasa Emany, Chittaranjan Bogishetty\",\"doi\":\"10.4103/jips.jips_194_25\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>Enhancing early cell-implant interaction is vital for successful osseointegration. This in vitro study aimed to evaluate the combined effect of photofunctionalization and low-frequency mechanical vibration on osteoblast cells derived from human placental tissue cultured on titanium surfaces.</p><p><strong>Settings and design: </strong>Following approval from the Institutional Ethics Committee and informed consent, a controlled in vitro experimental study was conducted in the Department of Prosthodontics in collaboration with a certified cell culture laboratory.</p><p><strong>Materials and methods: </strong>Osteoblast cells were isolated and cultured. Titanium implant surface was photofunctionalized using ultraviolet C (UV-C) light (254 nm) for 48 h. Cells were seeded onto the implant surface and divided into three groups: Group 1: Control (untreated titanium), Group 2: UV-treated titanium, and Group 3: UV-treated titanium implant with mechanical vibration (20 Hz for 3 min/day for 3 days) to osteoblast cells. Cell proliferation was assessed using MTT assay, mineralization via Alizarin Red S staining, and osteogenic gene expression (runt-related transcription factor-2, Alkaline phosphatase, OCN) through reverse transcription polymerase chain reaction.</p><p><strong>Statistical analysis used: </strong>Data were analyzed using analysis of variance with Tukey's post hoc test (P < 0.05).</p><p><strong>Results: </strong>The combination of photofunctionalization and mechanical vibration (Group 3) significantly enhanced cell proliferation, mineral deposition, and osteogenic gene expression compared to Groups 1 and 2 (P < 0.05). While photofunctionalization alone improved osteoblast activity, the synergistic application of mechanical vibration further amplified the response.</p><p><strong>Conclusion: </strong>The combination of photofunctionalization and low-frequency vibration significantly enhances the osteogenic response of osteoblast cells to titanium, indicating its potential to improve implant osseointegration.</p>\",\"PeriodicalId\":22669,\"journal\":{\"name\":\"The Journal of Indian Prosthodontic Society\",\"volume\":\"25 4\",\"pages\":\"334-339\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Indian Prosthodontic Society\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jips.jips_194_25\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/10/20 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Indian Prosthodontic Society","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jips.jips_194_25","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/10/20 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Effect of photofunctionalization with and without vibration on osseointegration of titanium implants: A comparative in vitro study.
Aim: Enhancing early cell-implant interaction is vital for successful osseointegration. This in vitro study aimed to evaluate the combined effect of photofunctionalization and low-frequency mechanical vibration on osteoblast cells derived from human placental tissue cultured on titanium surfaces.
Settings and design: Following approval from the Institutional Ethics Committee and informed consent, a controlled in vitro experimental study was conducted in the Department of Prosthodontics in collaboration with a certified cell culture laboratory.
Materials and methods: Osteoblast cells were isolated and cultured. Titanium implant surface was photofunctionalized using ultraviolet C (UV-C) light (254 nm) for 48 h. Cells were seeded onto the implant surface and divided into three groups: Group 1: Control (untreated titanium), Group 2: UV-treated titanium, and Group 3: UV-treated titanium implant with mechanical vibration (20 Hz for 3 min/day for 3 days) to osteoblast cells. Cell proliferation was assessed using MTT assay, mineralization via Alizarin Red S staining, and osteogenic gene expression (runt-related transcription factor-2, Alkaline phosphatase, OCN) through reverse transcription polymerase chain reaction.
Statistical analysis used: Data were analyzed using analysis of variance with Tukey's post hoc test (P < 0.05).
Results: The combination of photofunctionalization and mechanical vibration (Group 3) significantly enhanced cell proliferation, mineral deposition, and osteogenic gene expression compared to Groups 1 and 2 (P < 0.05). While photofunctionalization alone improved osteoblast activity, the synergistic application of mechanical vibration further amplified the response.
Conclusion: The combination of photofunctionalization and low-frequency vibration significantly enhances the osteogenic response of osteoblast cells to titanium, indicating its potential to improve implant osseointegration.
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