波斯尼亚和黑塞哥维那蚊子的横截面：条形码，血餐和病原体

IF 4.5 2区医学 Q1 INFECTIOUS DISEASES

One Health Pub Date : 2025-10-15 DOI:10.1016/j.onehlt.2025.101246

Ina Hoxha , Jovana Dervović , Maria Sophia Unterköfler , Lisa Schlamadinger , Tanto Situmorang , Hans-Peter Fuehrer , Adelheid G. Obwaller , Karin Sekulin , Jeremy V. Camp , Josef Harl , Julia Walochnik , Amer Alić , Edwin Kniha

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引用次数: 0

摘要

蚊子是人类和动物病原体的重要媒介，但在巴尔干中部国家波斯尼亚和黑塞哥维那，关于其多样性和媒介潜力的数据仍然很少。本研究旨在评估波黑地区的蚊子种类组成、相关病原体和潜在的公共卫生风险。采用灯诱法采集成蚊，进行形态鉴定和条形码鉴定，并对西尼罗病毒（WNV）、dirofilia、Trypanosoma、Plasmodium等多种病原菌进行分子筛选，共鉴定出17种2691只，其中以淡纹库蚊/torrentium数量最多，白纹伊蚊和伊蚊新记录。多糖类化合物对虾。波黑首次在蚊子中检测到西尼罗河病毒（2系）RNA，这与邻国的发现一致，突出了该地区传播的潜在风险。此外，还检测到丝状线虫（免疫Dirofilaria immitis， D. repens和苔原尾丝虫）的DNA，强调了它们作为人畜共患Dirofilaria tundra的潜在媒介的作用。此外，还检测到锥虫病和疟原虫DNA，需要进一步调查蚊子可能参与其传播。入侵伊蚊种类和蚊媒病原体的发现强调需要在东南欧加强媒介监测，特别是在波黑。本研究提供了波黑地区17种蚊子的第一个条形码清单和蚊媒病原体的新分子证据，为未来的流行病学评估和持续的昆虫学监测提供了有价值的基线数据。

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A cross section through mosquitoes of Bosnia and Herzegovina: Barcodes, blood meals and pathogens

Mosquitoes are important vectors of human and animal pathogens, yet data on their diversity and vector potential remain scarce for the central Balkan country Bosnia and Herzegovina. This study aimed to assess mosquito species composition, associated pathogens, and the potential public health risks in BIH. Adult mosquitoes were collected with light traps, identified morphologically and by barcoding, and screened molecularly for various pathogens, including West Nile virus (WNV), Dirofilaria spp., Trypanosoma spp., and Plasmodium spp.

A total of 2691 mosquitoes of 17 species were identified, with Culex pipiens/torrentium being most abundant and new records of Aedes albopictus and Ae. japonicus japonicus. The first detection of WNV (lineage 2) RNA in mosquitoes in BIH highlights the potential risk of circulation in the region, aligning with findings from neighboring countries. In addition, DNA of filarioid nematodes (Dirofilaria immitis, D. repens, and Setaria tundra) were detected, underscoring their role as potential vectors of zoonotic dirofilariasis. Also, Trypanosoma and Plasmodium DNA was detected, warranting further investigation into the possible involvement of mosquitoes in their transmission.

The detection of invasive Aedes species and mosquito-borne pathogens emphasize the need for strengthened vector surveillance in southeastern Europe, particularly in BIH. This study provides the first barcode inventory of 17 mosquito species and novel molecular evidence of mosquito-borne pathogens in BIH, offering valuable baseline data for future epidemiological assessments and sustained entomological surveillance.

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来源期刊

One Health Medicine-Infectious Diseases

CiteScore

8.10

自引率

4.00%

发文量

审稿时长

18 weeks

期刊介绍： One Health - a Gold Open Access journal. The mission of One Health is to provide a platform for rapid communication of high quality scientific knowledge on inter- and intra-species pathogen transmission, bringing together leading experts in virology, bacteriology, parasitology, mycology, vectors and vector-borne diseases, tropical health, veterinary sciences, pathology, immunology, food safety, mathematical modelling, epidemiology, public health research and emergency preparedness. As a Gold Open Access journal, a fee is payable on acceptance of the paper. Please see the Guide for Authors for more information. Submissions to the following categories are welcome: Virology, Bacteriology, Parasitology, Mycology, Vectors and vector-borne diseases, Co-infections and co-morbidities, Disease spatial surveillance, Modelling, Tropical Health, Discovery, Ecosystem Health, Public Health.