Natalia Méndez Acosta, Gabriela Rivas Alén, Ismael Rodriguez Grecco, Allexandra Díaz, Dalia Moreno, Ludwig R Frontier Ramos
{"title":"顶顶法和顶底法制备血液成分的比较评价：乌拉圭的变化。","authors":"Natalia Méndez Acosta, Gabriela Rivas Alén, Ismael Rodriguez Grecco, Allexandra Díaz, Dalia Moreno, Ludwig R Frontier Ramos","doi":"10.1111/tme.70039","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Hospital de Clínicas, in conjunction with the Universidad de la República UDELAR in Uruguay, evaluated the quality of blood components after separation with a top-and-bottom (TB) system, comparing it with the top-and-top (TT) system and verifying compliance with local and international standards.</p><p><strong>Study design and methods: </strong>Whole blood (WB) was collected using TB (n = 150) and TT (n = 130). TB units were processed with MacoPress Smart, obtaining red cell concentrate (RCC), plasma (PL), and buffy coat (BC). After 2 h, BC had a soft spin centrifugation for a single unit of platelet concentrate (SUPC). TT bags were managed on day 0 (up to 8 h of collection) with manual press obtaining RCC and platelet-rich plasma units (PRP). PRP had hard spin centrifugation and was separated again with the manual press to collect PL and SUPC.</p><p><strong>Results: </strong>TB system produced RCC with higher haemoglobin concentration and Haematocrit but with lower volume and Hb/unit (p < 0.001). The TB system produced RCC with lower leukocytes and residual platelet levels (p < 0.001). SUPC showed fewer residual leukocytes and red cell levels with TB (p < 0.001), but TT produced a higher mean platelet count than the TB system (p < 0.001). During this evaluation, there was no significant difference in the mean plasma volume (p = 0.178).</p><p><strong>Conclusion: </strong>The analysis favors the implementation of TB in the HC Blood Bank, improving the quality and safety of the transfusion service.</p>","PeriodicalId":23306,"journal":{"name":"Transfusion Medicine","volume":" ","pages":""},"PeriodicalIF":1.4000,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative evaluation of blood component preparation with the top-and-top and top-and-bottom methods: A change in Uruguay.\",\"authors\":\"Natalia Méndez Acosta, Gabriela Rivas Alén, Ismael Rodriguez Grecco, Allexandra Díaz, Dalia Moreno, Ludwig R Frontier Ramos\",\"doi\":\"10.1111/tme.70039\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Hospital de Clínicas, in conjunction with the Universidad de la República UDELAR in Uruguay, evaluated the quality of blood components after separation with a top-and-bottom (TB) system, comparing it with the top-and-top (TT) system and verifying compliance with local and international standards.</p><p><strong>Study design and methods: </strong>Whole blood (WB) was collected using TB (n = 150) and TT (n = 130). TB units were processed with MacoPress Smart, obtaining red cell concentrate (RCC), plasma (PL), and buffy coat (BC). After 2 h, BC had a soft spin centrifugation for a single unit of platelet concentrate (SUPC). TT bags were managed on day 0 (up to 8 h of collection) with manual press obtaining RCC and platelet-rich plasma units (PRP). PRP had hard spin centrifugation and was separated again with the manual press to collect PL and SUPC.</p><p><strong>Results: </strong>TB system produced RCC with higher haemoglobin concentration and Haematocrit but with lower volume and Hb/unit (p < 0.001). The TB system produced RCC with lower leukocytes and residual platelet levels (p < 0.001). SUPC showed fewer residual leukocytes and red cell levels with TB (p < 0.001), but TT produced a higher mean platelet count than the TB system (p < 0.001). During this evaluation, there was no significant difference in the mean plasma volume (p = 0.178).</p><p><strong>Conclusion: </strong>The analysis favors the implementation of TB in the HC Blood Bank, improving the quality and safety of the transfusion service.</p>\",\"PeriodicalId\":23306,\"journal\":{\"name\":\"Transfusion Medicine\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2025-10-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Transfusion Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/tme.70039\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transfusion Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/tme.70039","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}
Comparative evaluation of blood component preparation with the top-and-top and top-and-bottom methods: A change in Uruguay.
Background: Hospital de Clínicas, in conjunction with the Universidad de la República UDELAR in Uruguay, evaluated the quality of blood components after separation with a top-and-bottom (TB) system, comparing it with the top-and-top (TT) system and verifying compliance with local and international standards.
Study design and methods: Whole blood (WB) was collected using TB (n = 150) and TT (n = 130). TB units were processed with MacoPress Smart, obtaining red cell concentrate (RCC), plasma (PL), and buffy coat (BC). After 2 h, BC had a soft spin centrifugation for a single unit of platelet concentrate (SUPC). TT bags were managed on day 0 (up to 8 h of collection) with manual press obtaining RCC and platelet-rich plasma units (PRP). PRP had hard spin centrifugation and was separated again with the manual press to collect PL and SUPC.
Results: TB system produced RCC with higher haemoglobin concentration and Haematocrit but with lower volume and Hb/unit (p < 0.001). The TB system produced RCC with lower leukocytes and residual platelet levels (p < 0.001). SUPC showed fewer residual leukocytes and red cell levels with TB (p < 0.001), but TT produced a higher mean platelet count than the TB system (p < 0.001). During this evaluation, there was no significant difference in the mean plasma volume (p = 0.178).
Conclusion: The analysis favors the implementation of TB in the HC Blood Bank, improving the quality and safety of the transfusion service.
期刊介绍：
Transfusion Medicine publishes articles on transfusion medicine in its widest context, including blood transfusion practice (blood procurement, pharmaceutical, clinical, scientific, computing and documentary aspects), immunohaematology, immunogenetics, histocompatibility, medico-legal applications, and related molecular biology and biotechnology.
In addition to original articles, which may include brief communications and case reports, the journal contains a regular educational section (based on invited reviews and state-of-the-art reports), technical section (including quality assurance and current practice guidelines), leading articles, letters to the editor, occasional historical articles and signed book reviews. Some lectures from Society meetings that are likely to be of general interest to readers of the Journal may be published at the discretion of the Editor and subject to the availability of space in the Journal.
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