Laila S Almassri, Joshua C Harris, Kristen M Crane, Andrew P Ohl, Nick J Tokar, Brett R Schofield, Jesse W Young, Jeffrey G Mellott
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The objective of this study was to describe inhibitory Neuropeptide Y (NPY) synapses in the central nucleus of the IC.</p><p><strong>Method: </strong>We used electron microscopy (EM) and post-embedding anti-NPY immunogold histochemistry on tissue from adult Fischer Brown Norway rats. Inhibitory NPY synapses were identified by pleomorphic vesicles, symmetric synaptic junctions, and NPY-immunopositive presynaptic boutons.</p><p><strong>Results: </strong>The data demonstrate that NPY terminals largely (∼75 %) form symmetric synapses with pleomorphic vesicles, mostly target small and medium dendrites, and have larger average bouton areas, active zone lengths, and vesicle pools, as compared to the overall GABAergic population.</p><p><strong>Discussion: </strong>The subset of GABAergic neurons that co-express NPY feature synaptic characteristics suggesting that NPY neuromodulation has a widespread role in regulating excitation in the ICc.</p>","PeriodicalId":19254,"journal":{"name":"Neuropeptides","volume":"114 ","pages":"102566"},"PeriodicalIF":2.7000,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ultrastructural characterization of neuropeptide Y synapses in the central inferior colliculus of the Fischer Brown Norway rat.\",\"authors\":\"Laila S Almassri, Joshua C Harris, Kristen M Crane, Andrew P Ohl, Nick J Tokar, Brett R Schofield, Jesse W Young, Jeffrey G Mellott\",\"doi\":\"10.1016/j.npep.2025.102566\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The central nucleus of the inferior colliculus (ICc) is the primary target for ascending auditory projections from the lower auditory brainstem nuclei and the main source of ascending projections to the auditory thalamus. The ICc has an extensive network of tonotopically organized neurons, whose classification is a subject of broad interest. In order to understand the extensive microcircuitry of the ICc, it is important to ascertain the synaptic arrangements among molecularly identified neuronal populations. The objective of this study was to describe inhibitory Neuropeptide Y (NPY) synapses in the central nucleus of the IC.</p><p><strong>Method: </strong>We used electron microscopy (EM) and post-embedding anti-NPY immunogold histochemistry on tissue from adult Fischer Brown Norway rats. Inhibitory NPY synapses were identified by pleomorphic vesicles, symmetric synaptic junctions, and NPY-immunopositive presynaptic boutons.</p><p><strong>Results: </strong>The data demonstrate that NPY terminals largely (∼75 %) form symmetric synapses with pleomorphic vesicles, mostly target small and medium dendrites, and have larger average bouton areas, active zone lengths, and vesicle pools, as compared to the overall GABAergic population.</p><p><strong>Discussion: </strong>The subset of GABAergic neurons that co-express NPY feature synaptic characteristics suggesting that NPY neuromodulation has a widespread role in regulating excitation in the ICc.</p>\",\"PeriodicalId\":19254,\"journal\":{\"name\":\"Neuropeptides\",\"volume\":\"114 \",\"pages\":\"102566\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-10-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Neuropeptides\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.npep.2025.102566\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuropeptides","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.npep.2025.102566","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
Ultrastructural characterization of neuropeptide Y synapses in the central inferior colliculus of the Fischer Brown Norway rat.
Introduction: The central nucleus of the inferior colliculus (ICc) is the primary target for ascending auditory projections from the lower auditory brainstem nuclei and the main source of ascending projections to the auditory thalamus. The ICc has an extensive network of tonotopically organized neurons, whose classification is a subject of broad interest. In order to understand the extensive microcircuitry of the ICc, it is important to ascertain the synaptic arrangements among molecularly identified neuronal populations. The objective of this study was to describe inhibitory Neuropeptide Y (NPY) synapses in the central nucleus of the IC.
Method: We used electron microscopy (EM) and post-embedding anti-NPY immunogold histochemistry on tissue from adult Fischer Brown Norway rats. Inhibitory NPY synapses were identified by pleomorphic vesicles, symmetric synaptic junctions, and NPY-immunopositive presynaptic boutons.
Results: The data demonstrate that NPY terminals largely (∼75 %) form symmetric synapses with pleomorphic vesicles, mostly target small and medium dendrites, and have larger average bouton areas, active zone lengths, and vesicle pools, as compared to the overall GABAergic population.
Discussion: The subset of GABAergic neurons that co-express NPY feature synaptic characteristics suggesting that NPY neuromodulation has a widespread role in regulating excitation in the ICc.
期刊介绍:
The aim of Neuropeptides is the rapid publication of original research and review articles, dealing with the structure, distribution, actions and functions of peptides in the central and peripheral nervous systems. The explosion of research activity in this field has led to the identification of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems.
The journal provides a unique opportunity of integrating the many disciplines involved in all neuropeptide research. The journal publishes articles on all aspects of the neuropeptide field, with particular emphasis on gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.