{"title":"将森林登革热病毒2型（DENV-2）感染系统错误分类为“未确定血清型”：在常规RT-qPCR监测中的意义","authors":"Idrissa Dieng","doi":"10.1186/s41182-025-00827-0","DOIUrl":null,"url":null,"abstract":"<p><p>Recent findings by Suppiah et al. revealed DENV-2 positive samples misclassified as \"Undetermined Serotype\" when using commercial dengue molecular serotyping assays. This highlights the noninclusion and resulting misclassification of this viral strain as a dengue virus serotype using available molecular tools. Drawing parallels from the notification of the same assay failure against sylvatic DENV-2 strains in Senegal, West Africa, this correspondence advocates for updating the existing tools to account for this viral variant. Development of multiplex assays including both urban and sylvatic strains may enhance dengue virus surveillance and provide real time monitoring of the prevalence of sylvatic viral lineages among recorded dengue infections in surveillance data.</p>","PeriodicalId":23311,"journal":{"name":"Tropical Medicine and Health","volume":"53 1","pages":"138"},"PeriodicalIF":3.5000,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12529781/pdf/","citationCount":"0","resultStr":"{\"title\":\"Systematic misclassification of sylvatic dengue virus 2 (DENV-2) infections as \\\"undetermined serotype\\\": implications in routine RT-qPCR surveillance.\",\"authors\":\"Idrissa Dieng\",\"doi\":\"10.1186/s41182-025-00827-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Recent findings by Suppiah et al. revealed DENV-2 positive samples misclassified as \\\"Undetermined Serotype\\\" when using commercial dengue molecular serotyping assays. This highlights the noninclusion and resulting misclassification of this viral strain as a dengue virus serotype using available molecular tools. Drawing parallels from the notification of the same assay failure against sylvatic DENV-2 strains in Senegal, West Africa, this correspondence advocates for updating the existing tools to account for this viral variant. Development of multiplex assays including both urban and sylvatic strains may enhance dengue virus surveillance and provide real time monitoring of the prevalence of sylvatic viral lineages among recorded dengue infections in surveillance data.</p>\",\"PeriodicalId\":23311,\"journal\":{\"name\":\"Tropical Medicine and Health\",\"volume\":\"53 1\",\"pages\":\"138\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12529781/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical Medicine and Health\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s41182-025-00827-0\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"TROPICAL MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Medicine and Health","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s41182-025-00827-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"TROPICAL MEDICINE","Score":null,"Total":0}
Systematic misclassification of sylvatic dengue virus 2 (DENV-2) infections as "undetermined serotype": implications in routine RT-qPCR surveillance.
Recent findings by Suppiah et al. revealed DENV-2 positive samples misclassified as "Undetermined Serotype" when using commercial dengue molecular serotyping assays. This highlights the noninclusion and resulting misclassification of this viral strain as a dengue virus serotype using available molecular tools. Drawing parallels from the notification of the same assay failure against sylvatic DENV-2 strains in Senegal, West Africa, this correspondence advocates for updating the existing tools to account for this viral variant. Development of multiplex assays including both urban and sylvatic strains may enhance dengue virus surveillance and provide real time monitoring of the prevalence of sylvatic viral lineages among recorded dengue infections in surveillance data.
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