{"title":"亲水相互作用液相色谱在中药分离分析中的应用进展。","authors":"Yuanyuan Wu, Hui Huang, Xianglong Zhao, Junhao Lin, Jialiang Guo, Zhengjin Jiang, Kesara Ar-Sanork, Tao Deng, Dongsheng Xu","doi":"10.3389/fchem.2025.1694998","DOIUrl":null,"url":null,"abstract":"<p><p>Traditional Chinese medicine (TCM), with a history spanning over 2,000 years, contains numerous strongly polar components that are increasingly the focus of pharmaceutical and analytical research. However, conventional reversed-phase liquid chromatography usually fails to adequately retain and separate these highly polar components. Hydrophilic interaction liquid chromatography (HILIC) has emerged as a powerful complementary technique, offering improved retention and separation for polar analytes. In recent years, HILIC has seen rapid development and wide application in the analysis of TCM. This review summarizes the major types of HILIC stationary phases and their recent applications in the separation of polar components in TCM. Particular attention is given to the analysis, quality control, and metabolomic profiling of bioactive compounds such as flavonoids, alkaloids, and saponins, highlighting the increasingly important role of HILIC in promoting TCM research.</p>","PeriodicalId":12421,"journal":{"name":"Frontiers in Chemistry","volume":"13 ","pages":"1694998"},"PeriodicalIF":4.2000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521266/pdf/","citationCount":"0","resultStr":"{\"title\":\"Recent progress in the application of hydrophilic interaction liquid chromatography for the separation and analysis of traditional Chinese medicine.\",\"authors\":\"Yuanyuan Wu, Hui Huang, Xianglong Zhao, Junhao Lin, Jialiang Guo, Zhengjin Jiang, Kesara Ar-Sanork, Tao Deng, Dongsheng Xu\",\"doi\":\"10.3389/fchem.2025.1694998\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Traditional Chinese medicine (TCM), with a history spanning over 2,000 years, contains numerous strongly polar components that are increasingly the focus of pharmaceutical and analytical research. However, conventional reversed-phase liquid chromatography usually fails to adequately retain and separate these highly polar components. Hydrophilic interaction liquid chromatography (HILIC) has emerged as a powerful complementary technique, offering improved retention and separation for polar analytes. In recent years, HILIC has seen rapid development and wide application in the analysis of TCM. This review summarizes the major types of HILIC stationary phases and their recent applications in the separation of polar components in TCM. Particular attention is given to the analysis, quality control, and metabolomic profiling of bioactive compounds such as flavonoids, alkaloids, and saponins, highlighting the increasingly important role of HILIC in promoting TCM research.</p>\",\"PeriodicalId\":12421,\"journal\":{\"name\":\"Frontiers in Chemistry\",\"volume\":\"13 \",\"pages\":\"1694998\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521266/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.3389/fchem.2025.1694998\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.3389/fchem.2025.1694998","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Recent progress in the application of hydrophilic interaction liquid chromatography for the separation and analysis of traditional Chinese medicine.
Traditional Chinese medicine (TCM), with a history spanning over 2,000 years, contains numerous strongly polar components that are increasingly the focus of pharmaceutical and analytical research. However, conventional reversed-phase liquid chromatography usually fails to adequately retain and separate these highly polar components. Hydrophilic interaction liquid chromatography (HILIC) has emerged as a powerful complementary technique, offering improved retention and separation for polar analytes. In recent years, HILIC has seen rapid development and wide application in the analysis of TCM. This review summarizes the major types of HILIC stationary phases and their recent applications in the separation of polar components in TCM. Particular attention is given to the analysis, quality control, and metabolomic profiling of bioactive compounds such as flavonoids, alkaloids, and saponins, highlighting the increasingly important role of HILIC in promoting TCM research.
期刊介绍：
Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide.
Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”.
All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
