Wenqun Jiang, Weidong Chen, Shihao Lin, Pinpin Hou, Yichao Jin, Xiaohua Zhang, Hui Wu, Li Gao, Qin Hu
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Functional enrichment analysis and competing endogenous RNA (ceRNA) network construction were conducted to explore potential regulatory mechanisms.</p><p><strong>Results: </strong>Two circRNAs, hsa_circ_0075436 and hsa_circ_0005729, significantly decreased in PBMCs of AIS patients. The expressive levels negatively correlated with NIH Stroke Scale (NIHSS) scores at admission and discharge. Receiver operating characteristic (ROC) curve analysis demonstrated their strong diagnostic performance. Bioinformatics analyses and qRT-PCR further suggested that hsa_circ_0005729 may influence BBB disruption and peripheral immune suppression in AIS via hsa-miR-1301/COL1A1 axis.</p><p><strong>Conclusion: </strong>Hsa_circ_0075436 and hsa_circ_0005729 are promising PBMC-derived biomarkers for the diagnosis and prognosis of AIS.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1657284"},"PeriodicalIF":3.9000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12520956/pdf/","citationCount":"0","resultStr":"{\"title\":\"Peripheral circular RNAs hsa_circ_0075436 and hsa_circ_0005729 as diagnostic and prognostic biomarkers in acute ischemic stroke: expression profiles and mechanistic insights.\",\"authors\":\"Wenqun Jiang, Weidong Chen, Shihao Lin, Pinpin Hou, Yichao Jin, Xiaohua Zhang, Hui Wu, Li Gao, Qin Hu\",\"doi\":\"10.3389/fmolb.2025.1657284\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Circular RNAs (circRNAs) are a class of stable, conserved, and tissue-specific non-coding RNAs that have shown potential as diagnostic and prognostic biomarkers in acute ischemic stroke (AIS). However, their expression and functional roles in peripheral blood mononuclear cells (PBMCs) remain largely unexplored.</p><p><strong>Methods: </strong>We performed whole transcriptome RNA sequencing on PBMC samples from AIS patients (n = 5) and matched healthy controls (n = 5). The top 10 differentially expressed circRNAs were validated by qRT-PCR in a validation cohort (n = 60), and two consistently dysregulated circRNAs were further validated in a larger replication cohort (n = 288). Functional enrichment analysis and competing endogenous RNA (ceRNA) network construction were conducted to explore potential regulatory mechanisms.</p><p><strong>Results: </strong>Two circRNAs, hsa_circ_0075436 and hsa_circ_0005729, significantly decreased in PBMCs of AIS patients. The expressive levels negatively correlated with NIH Stroke Scale (NIHSS) scores at admission and discharge. Receiver operating characteristic (ROC) curve analysis demonstrated their strong diagnostic performance. Bioinformatics analyses and qRT-PCR further suggested that hsa_circ_0005729 may influence BBB disruption and peripheral immune suppression in AIS via hsa-miR-1301/COL1A1 axis.</p><p><strong>Conclusion: </strong>Hsa_circ_0075436 and hsa_circ_0005729 are promising PBMC-derived biomarkers for the diagnosis and prognosis of AIS.</p>\",\"PeriodicalId\":12465,\"journal\":{\"name\":\"Frontiers in Molecular Biosciences\",\"volume\":\"12 \",\"pages\":\"1657284\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12520956/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Molecular Biosciences\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3389/fmolb.2025.1657284\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Molecular Biosciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fmolb.2025.1657284","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Peripheral circular RNAs hsa_circ_0075436 and hsa_circ_0005729 as diagnostic and prognostic biomarkers in acute ischemic stroke: expression profiles and mechanistic insights.
Introduction: Circular RNAs (circRNAs) are a class of stable, conserved, and tissue-specific non-coding RNAs that have shown potential as diagnostic and prognostic biomarkers in acute ischemic stroke (AIS). However, their expression and functional roles in peripheral blood mononuclear cells (PBMCs) remain largely unexplored.
Methods: We performed whole transcriptome RNA sequencing on PBMC samples from AIS patients (n = 5) and matched healthy controls (n = 5). The top 10 differentially expressed circRNAs were validated by qRT-PCR in a validation cohort (n = 60), and two consistently dysregulated circRNAs were further validated in a larger replication cohort (n = 288). Functional enrichment analysis and competing endogenous RNA (ceRNA) network construction were conducted to explore potential regulatory mechanisms.
Results: Two circRNAs, hsa_circ_0075436 and hsa_circ_0005729, significantly decreased in PBMCs of AIS patients. The expressive levels negatively correlated with NIH Stroke Scale (NIHSS) scores at admission and discharge. Receiver operating characteristic (ROC) curve analysis demonstrated their strong diagnostic performance. Bioinformatics analyses and qRT-PCR further suggested that hsa_circ_0005729 may influence BBB disruption and peripheral immune suppression in AIS via hsa-miR-1301/COL1A1 axis.
Conclusion: Hsa_circ_0075436 and hsa_circ_0005729 are promising PBMC-derived biomarkers for the diagnosis and prognosis of AIS.
期刊介绍：
Much of contemporary investigation in the life sciences is devoted to the molecular-scale understanding of the relationships between genes and the environment — in particular, dynamic alterations in the levels, modifications, and interactions of cellular effectors, including proteins. Frontiers in Molecular Biosciences offers an international publication platform for basic as well as applied research; we encourage contributions spanning both established and emerging areas of biology. To this end, the journal draws from empirical disciplines such as structural biology, enzymology, biochemistry, and biophysics, capitalizing as well on the technological advancements that have enabled metabolomics and proteomics measurements in massively parallel throughput, and the development of robust and innovative computational biology strategies. We also recognize influences from medicine and technology, welcoming studies in molecular genetics, molecular diagnostics and therapeutics, and nanotechnology.
Our ultimate objective is the comprehensive illustration of the molecular mechanisms regulating proteins, nucleic acids, carbohydrates, lipids, and small metabolites in organisms across all branches of life.
In addition to interesting new findings, techniques, and applications, Frontiers in Molecular Biosciences will consider new testable hypotheses to inspire different perspectives and stimulate scientific dialogue. The integration of in silico, in vitro, and in vivo approaches will benefit endeavors across all domains of the life sciences.
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