Ting Mo, Hong Yu Ren, Xian Xian Zhang, Yun Wei Lu, Zhong Qiu Teng, Xue Zhang, Lu Peng Dai, Ling Hou, Na Zhao, Jia He, Tian Qin
{"title":"嗜肺军团菌I-F型CRISPR-Cas的表型功能。","authors":"Ting Mo, Hong Yu Ren, Xian Xian Zhang, Yun Wei Lu, Zhong Qiu Teng, Xue Zhang, Lu Peng Dai, Ling Hou, Na Zhao, Jia He, Tian Qin","doi":"10.3967/bes2025.107","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>CRISPR-Cas protects bacteria from exogenous DNA invasion and is associated with bacterial biofilm formation and pathogenicity.</p><p><strong>Methods: </strong>We analyzed the type I-F CRISPR-Cas system of <i>Legionella pneumophila</i> WX48, including <i>Cas1, Cas2-Cas3, Csy1, Csy2, Csy3</i>, and <i>Cas6f</i>, along with downstream CRISPR arrays. We explored the effects of the CRISPR-Cas system on the in vitro growth, biofilm-forming ability, and pathogenicity of <i>L. pneumophila</i> through constructing gene deletion mutants.</p><p><strong>Results: </strong>The type I-F CRISPR-Cas system did not affect the <i>in vitro</i> growth of wild-type or mutant strains. The biofilm formation and intracellular proliferation of the mutant strains were weaker than those of the wild type owing to the regulation of type IV pili and Dot/Icm type IV secretion systems. In particular, <i>Cas6f</i> deletion strongly inhibited these processes.</p><p><strong>Conclusion: </strong>The type I-F CRISPR-Cas system may reduce biofilm formation and intracellular proliferation in <i>L. pneumophila</i>.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 9","pages":"1105-1119"},"PeriodicalIF":4.1000,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phenotypic Function of <i>Legionella pneumophila</i> Type I-F CRISPR-Cas.\",\"authors\":\"Ting Mo, Hong Yu Ren, Xian Xian Zhang, Yun Wei Lu, Zhong Qiu Teng, Xue Zhang, Lu Peng Dai, Ling Hou, Na Zhao, Jia He, Tian Qin\",\"doi\":\"10.3967/bes2025.107\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>CRISPR-Cas protects bacteria from exogenous DNA invasion and is associated with bacterial biofilm formation and pathogenicity.</p><p><strong>Methods: </strong>We analyzed the type I-F CRISPR-Cas system of <i>Legionella pneumophila</i> WX48, including <i>Cas1, Cas2-Cas3, Csy1, Csy2, Csy3</i>, and <i>Cas6f</i>, along with downstream CRISPR arrays. We explored the effects of the CRISPR-Cas system on the in vitro growth, biofilm-forming ability, and pathogenicity of <i>L. pneumophila</i> through constructing gene deletion mutants.</p><p><strong>Results: </strong>The type I-F CRISPR-Cas system did not affect the <i>in vitro</i> growth of wild-type or mutant strains. The biofilm formation and intracellular proliferation of the mutant strains were weaker than those of the wild type owing to the regulation of type IV pili and Dot/Icm type IV secretion systems. In particular, <i>Cas6f</i> deletion strongly inhibited these processes.</p><p><strong>Conclusion: </strong>The type I-F CRISPR-Cas system may reduce biofilm formation and intracellular proliferation in <i>L. pneumophila</i>.</p>\",\"PeriodicalId\":93903,\"journal\":{\"name\":\"Biomedical and environmental sciences : BES\",\"volume\":\"38 9\",\"pages\":\"1105-1119\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2025-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical and environmental sciences : BES\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3967/bes2025.107\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical and environmental sciences : BES","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3967/bes2025.107","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Phenotypic Function of Legionella pneumophila Type I-F CRISPR-Cas.
Objective: CRISPR-Cas protects bacteria from exogenous DNA invasion and is associated with bacterial biofilm formation and pathogenicity.
Methods: We analyzed the type I-F CRISPR-Cas system of Legionella pneumophila WX48, including Cas1, Cas2-Cas3, Csy1, Csy2, Csy3, and Cas6f, along with downstream CRISPR arrays. We explored the effects of the CRISPR-Cas system on the in vitro growth, biofilm-forming ability, and pathogenicity of L. pneumophila through constructing gene deletion mutants.
Results: The type I-F CRISPR-Cas system did not affect the in vitro growth of wild-type or mutant strains. The biofilm formation and intracellular proliferation of the mutant strains were weaker than those of the wild type owing to the regulation of type IV pili and Dot/Icm type IV secretion systems. In particular, Cas6f deletion strongly inhibited these processes.
Conclusion: The type I-F CRISPR-Cas system may reduce biofilm formation and intracellular proliferation in L. pneumophila.
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