Effect of proanthocyanidins application on push-out bond strength of root canal filling after different final irrigation procedures.

Background: This study aims to investigate the effects of using EDTA, phytic acid (IP6), chitosan, and proanthocyanidin (PAs) as final irrigation solutions on the push-out bond strength (PBS) of root canal filling.

Materials methods: In the present study, 56 extracted human incisors were utilized. All teeth were decoronated at the cementoenamel junction. Following the root canal chemomechanical preparation, the teeth were randomly divided into 7 groups according to the final irrigation procedures: Group 1: Control, Group 2: EDTA, Group 3: IP6, Group 4: Chitosan, Group 5: EDTA + PAs, Group 6: IP6 + PAs, Group 7: Chitosan + PAs. The root canals were filled using the cold lateral condensation technique with gutta-percha and resin-based root canal sealer. Samples were obtained horizontally cut from the apical third of the teeth and subjected to push-out bond strength (PBS) using a universal testing machine. Data was statistically analyzed using one-way ANOVA and post hoc Tukey HSD test (α = 0.05).

Results: The highest value was obtained in Group 6 (12.14 ± 0.47 MPa), and the lowest value in Group 1 (5.80 ± 0,44 MPa) (P < 0.05). Group 6 has shown a higher PBS value than Group 5 (11.72 ± 0.14 MPa) and Group 7 (11.94 ± 0.42 MPa) (P > 0.05). There were statistically significant differences between all other groups, except Groups 3 and 4 (P < 0.05). The failure type was mostly adhesive, and no cohesive failures were observed.

Conclusion: The use of EDTA, IP6, and chitosan for final irrigation has enhanced the bond strength values of resin-based root canal sealer to dentin. Furthermore, the use of PAs after EDTA, IP6, and chitosan increased the bond strength values more than when these solutions were used alone.