Wei Sun, Xiaojia Wang, Chenfei Wang, Zhili Li, Rui Guo, Zhedong Ma, Li Luo, Ming Zeng, Dezhong Zhou
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Multifunctional and UV Light-Triggered Degradable Highly Branched Vinyl Polymer for Efficient mRNA Delivery.
Vinyl polymers synthesized via radical polymerization of (meth)acrylates are widely used in various biomedical fields. We developed a multifunctional, highly branched vinyl polymer, HPNDN, with ultraviolet (UV) light-triggered degradability for highly efficient mRNA delivery. (2-Nitro-1,3-phenylene) bis(methylene) diacrylate (NPBMDA) was copolymerized with 2-(dimethylamino)ethyl methacrylate (DMAEMA) and 3,3,4,4,5,5,6,6-nonafluorohexyl acrylate (NFHA) by reversible addition-fragmentation chain transfer (RAFT) polymerization followed by end-capping with ethylenediamine (EDA), resulting in HPNDN with a highly branched topological structure, multiple fluorine moieties, and multiple amines. Upon exposure to UV light irradiation, HPNDN degrades within 5 min through the cleavage of the ester groups in the NPBMDA units. Importantly, HPNDN can effectively condense mRNA to form nanosized polyplexes, achieving high transfection efficiency of up to 87.99%, 62.75% and 52.37% in African green monkey kidney (COS-7) cells, human embryonic kidney (293T) cells, and rat schwann (RSC96) cells, respectively, without inducing obvious cytotoxicity.
期刊介绍：
Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine.
Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.
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