滚入基因组：通过无标记全息细胞术将突变与细胞结构联系起来。

IF 23.4 Q1 OPTICS

Light-Science & Applications Pub Date : 2025-10-14 DOI:10.1038/s41377-025-02053-z

Maciej Trusiak

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引用次数: 0

摘要

证明了悬浮急性髓系白血病母细胞无标记表型的全息层析流式细胞术。在三维折射率层析图中应用凹分割算法来量化npm1突变相关的杯状核形态，虚拟现实可视化提供引人入胜的沉浸感。该方法能够在群体水平上检测3D细胞形态的统计学显著变化，最初将表型与基因型相关。

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Rolling into the genome: linking mutations to cellular structure through label-free holographic cytometry.

Holo-tomographic flow cytometry for label-free phenotyping of suspended acute myeloid leukemia blasts is demonstrated. A concave segmentation algorithm is applied to 3D refractive index tomograms to quantify NPM1-mutation-associated cup-like nuclear morphologies, with virtual reality visualization offering engaging immersion. The method enables population-level detection of statistically significant shifts in 3D cell morphology, originally correlating phenotype with genotype.

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来源期刊

Light-Science & Applications 数理科学, 物理学I, 光学, 凝聚态物性 II ：电子结构、电学、磁学和光学性质, 无机非金属材料, 无机非金属类光电信息与功能材料, 工程与材料, 信息科学, 光学和光电子学, 光学和光电子材料, 非线性光学与量子光学

自引率

0.00%

发文量

803

审稿时长

2.1 months