{"title":"靶向DNA甲基化与血栓形成的相关性研究。","authors":"Xiang Kui, Junfei Feng, Jing Leng, Cong Sun, Qiuheng Tang, Haifeng Li","doi":"10.1007/s12288-024-01936-2","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to investigate the relationship of leukocytes DNA methylation in targeted sites and thrombophilia.</p><p><strong>Methods: </strong>Eight thrombophilia patients and their kin-related individuals as the healthy control. Targeted DNA methylation from peripheral leukocytes were examined with MassArray. Multivariate correlation analysis was used to estimate targeted gene methylation as an independent risk factor of thrombophilia. Receiver operating characteristic curve analysis was used to calculate the accuracy of biomarkers in the prediction of thrombophilia.</p><p><strong>Results: </strong>The age of thrombophilia group was higher than control group (<i>P</i> < 0.001). F5.24.CpG.10 and Protein S.44.CpG.29-33 methylation were significantly associated with thrombophilia negatively and positively (<i>r</i> = -0.7289, <i>P</i> < 0.01 and <i>r</i> = 0.5667, <i>P</i> < 0.05). F5.24.CpG.10 methylation was higher in control group (<i>P</i> < 0.01), but Protein S.44.CpG.29-33 methylation increased in thrombophilia group (<i>P</i> < 0.05). The areas under curve of ROC were 0.9297 and 0.8437, respectively.</p><p><strong>Conclusion: </strong>Target DNA methylation in Protein S.44.CpG.29-33 island is associated with an elevated risk of thrombophilia.</p>","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 4","pages":"878-885"},"PeriodicalIF":0.6000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12515139/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Association Study of Targeted DNA Methylation and Thrombophilia.\",\"authors\":\"Xiang Kui, Junfei Feng, Jing Leng, Cong Sun, Qiuheng Tang, Haifeng Li\",\"doi\":\"10.1007/s12288-024-01936-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The aim of this study is to investigate the relationship of leukocytes DNA methylation in targeted sites and thrombophilia.</p><p><strong>Methods: </strong>Eight thrombophilia patients and their kin-related individuals as the healthy control. Targeted DNA methylation from peripheral leukocytes were examined with MassArray. Multivariate correlation analysis was used to estimate targeted gene methylation as an independent risk factor of thrombophilia. Receiver operating characteristic curve analysis was used to calculate the accuracy of biomarkers in the prediction of thrombophilia.</p><p><strong>Results: </strong>The age of thrombophilia group was higher than control group (<i>P</i> < 0.001). F5.24.CpG.10 and Protein S.44.CpG.29-33 methylation were significantly associated with thrombophilia negatively and positively (<i>r</i> = -0.7289, <i>P</i> < 0.01 and <i>r</i> = 0.5667, <i>P</i> < 0.05). F5.24.CpG.10 methylation was higher in control group (<i>P</i> < 0.01), but Protein S.44.CpG.29-33 methylation increased in thrombophilia group (<i>P</i> < 0.05). The areas under curve of ROC were 0.9297 and 0.8437, respectively.</p><p><strong>Conclusion: </strong>Target DNA methylation in Protein S.44.CpG.29-33 island is associated with an elevated risk of thrombophilia.</p>\",\"PeriodicalId\":49188,\"journal\":{\"name\":\"Indian Journal of Hematology and Blood Transfusion\",\"volume\":\"41 4\",\"pages\":\"878-885\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12515139/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Hematology and Blood Transfusion\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s12288-024-01936-2\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/11 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Hematology and Blood Transfusion","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12288-024-01936-2","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/11 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"HEMATOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的：探讨白细胞靶向位点DNA甲基化与血栓形成的关系。方法：选取8例血栓病患者及其亲属为健康对照。用MassArray检测外周白细胞的靶向DNA甲基化。使用多变量相关分析来估计靶向基因甲基化是血栓形成的独立危险因素。使用受试者工作特征曲线分析来计算生物标志物预测血栓性疾病的准确性。结果：血栓组患者年龄高于对照组（P r = -0.7289, Pr = 0.5667, P P P P）。结论：蛋白S.44.CpG靶DNA甲基化。29-33岛与血栓形成风险升高有关。
The Association Study of Targeted DNA Methylation and Thrombophilia.
Objective: The aim of this study is to investigate the relationship of leukocytes DNA methylation in targeted sites and thrombophilia.
Methods: Eight thrombophilia patients and their kin-related individuals as the healthy control. Targeted DNA methylation from peripheral leukocytes were examined with MassArray. Multivariate correlation analysis was used to estimate targeted gene methylation as an independent risk factor of thrombophilia. Receiver operating characteristic curve analysis was used to calculate the accuracy of biomarkers in the prediction of thrombophilia.
Results: The age of thrombophilia group was higher than control group (P < 0.001). F5.24.CpG.10 and Protein S.44.CpG.29-33 methylation were significantly associated with thrombophilia negatively and positively (r = -0.7289, P < 0.01 and r = 0.5667, P < 0.05). F5.24.CpG.10 methylation was higher in control group (P < 0.01), but Protein S.44.CpG.29-33 methylation increased in thrombophilia group (P < 0.05). The areas under curve of ROC were 0.9297 and 0.8437, respectively.
Conclusion: Target DNA methylation in Protein S.44.CpG.29-33 island is associated with an elevated risk of thrombophilia.
期刊介绍：
Indian Journal of Hematology and Blood Transfusion is a medium for propagating and exchanging ideas within the medical community. It publishes peer-reviewed articles on a variety of aspects of clinical hematology, laboratory hematology and hemato-oncology. The journal exists to encourage scientific investigation in the study of blood in health and in disease; to promote and foster the exchange and diffusion of knowledge relating to blood and blood-forming tissues; and to provide a forum for discussion of hematological subjects on a national scale.
The Journal is the official publication of The Indian Society of Hematology & Blood Transfusion.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
