Rodolfo M Ortiz-Flores, Alejandro Escamilla-Sanchez, Borja Cidoncha-Morcillo, Luis Mastronardi, Silvia Fontenete, Regina Garcia-Delgado
{"title":"增强面部年轻化：海兰凝胶真皮填充剂DX对胶原合成和组织再生的生物刺激作用。","authors":"Rodolfo M Ortiz-Flores, Alejandro Escamilla-Sanchez, Borja Cidoncha-Morcillo, Luis Mastronardi, Silvia Fontenete, Regina Garcia-Delgado","doi":"10.1007/s00266-025-05245-5","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Facial rejuvenation involves addressing the breakdown of collagen and elastin that occurs with aging. Hyaluronic acid (HA)-based dermal fillers have become a key non-invasive treatment, known for their volumizing effects and potential regenerative benefits. This study characterizes the physicochemical and rheological properties of Hylan Gel Dermal Filler DX, a novel filler combining HA and dextranomer microspheres, and evaluates its capacity to enhance extracellular matrix regeneration.</p><p><strong>Methods: </strong>Rheological analysis was conducted to determine viscoelastic properties, including storage modulus (G'), loss modulus (G''), and tan delta across frequencies (0.1-10 Hz). In vitro experiments with normal human dermal fibroblasts (NHDFs) and monocytes (J774A.1) assessed the effects of Hylan Gel Dermal Filler DX (HA + DX), Genefill Soft Fill (HA), HarmonyCa® (HA+CaHa), Radiesse® (CaHa), and Sculptra® (PLLA). Hydration and wound healing assays were also performed. Biomarker analysis focused on collagen types I, III, IV, VI, elastin, and cytokines (TGF-β1, IL-1β, IL-6, IL-10, TNFα).</p><p><strong>Results: </strong>Rheologically, HA + DX displayed a balanced elastic and viscous profile, supporting its structural integrity and adaptability in dynamic facial areas. HA + DX demonstrated high stimulation of collagen types I, III, IV, VI, and elastin production, with enhanced fibroblast migration and wound healing at 72 hours compared to other fillers. HA + DX also modulated cytokines, increasing TGF-β1 and IL-10 while reducing TNFα and IL-1β, suggesting anti-inflammatory and regenerative properties.</p><p><strong>Conclusions: </strong>HA + DX exhibited considerable benefits in collagen stimulation, wound healing, and cytokine modulation. Its favorable rheological characteristics and regenerative potential make it a promising option for facial rejuvenation and tissue regeneration.</p><p><strong>No level assigned: </strong>This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.</p>","PeriodicalId":7609,"journal":{"name":"Aesthetic Plastic Surgery","volume":" ","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced Facial Rejuvenation: Biostimulatory Effects of Hylan Gel Dermal Filler DX on Collagen Synthesis and Tissue Regeneration.\",\"authors\":\"Rodolfo M Ortiz-Flores, Alejandro Escamilla-Sanchez, Borja Cidoncha-Morcillo, Luis Mastronardi, Silvia Fontenete, Regina Garcia-Delgado\",\"doi\":\"10.1007/s00266-025-05245-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Facial rejuvenation involves addressing the breakdown of collagen and elastin that occurs with aging. Hyaluronic acid (HA)-based dermal fillers have become a key non-invasive treatment, known for their volumizing effects and potential regenerative benefits. This study characterizes the physicochemical and rheological properties of Hylan Gel Dermal Filler DX, a novel filler combining HA and dextranomer microspheres, and evaluates its capacity to enhance extracellular matrix regeneration.</p><p><strong>Methods: </strong>Rheological analysis was conducted to determine viscoelastic properties, including storage modulus (G'), loss modulus (G''), and tan delta across frequencies (0.1-10 Hz). In vitro experiments with normal human dermal fibroblasts (NHDFs) and monocytes (J774A.1) assessed the effects of Hylan Gel Dermal Filler DX (HA + DX), Genefill Soft Fill (HA), HarmonyCa® (HA+CaHa), Radiesse® (CaHa), and Sculptra® (PLLA). Hydration and wound healing assays were also performed. Biomarker analysis focused on collagen types I, III, IV, VI, elastin, and cytokines (TGF-β1, IL-1β, IL-6, IL-10, TNFα).</p><p><strong>Results: </strong>Rheologically, HA + DX displayed a balanced elastic and viscous profile, supporting its structural integrity and adaptability in dynamic facial areas. HA + DX demonstrated high stimulation of collagen types I, III, IV, VI, and elastin production, with enhanced fibroblast migration and wound healing at 72 hours compared to other fillers. HA + DX also modulated cytokines, increasing TGF-β1 and IL-10 while reducing TNFα and IL-1β, suggesting anti-inflammatory and regenerative properties.</p><p><strong>Conclusions: </strong>HA + DX exhibited considerable benefits in collagen stimulation, wound healing, and cytokine modulation. 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Enhanced Facial Rejuvenation: Biostimulatory Effects of Hylan Gel Dermal Filler DX on Collagen Synthesis and Tissue Regeneration.
Background: Facial rejuvenation involves addressing the breakdown of collagen and elastin that occurs with aging. Hyaluronic acid (HA)-based dermal fillers have become a key non-invasive treatment, known for their volumizing effects and potential regenerative benefits. This study characterizes the physicochemical and rheological properties of Hylan Gel Dermal Filler DX, a novel filler combining HA and dextranomer microspheres, and evaluates its capacity to enhance extracellular matrix regeneration.
Methods: Rheological analysis was conducted to determine viscoelastic properties, including storage modulus (G'), loss modulus (G''), and tan delta across frequencies (0.1-10 Hz). In vitro experiments with normal human dermal fibroblasts (NHDFs) and monocytes (J774A.1) assessed the effects of Hylan Gel Dermal Filler DX (HA + DX), Genefill Soft Fill (HA), HarmonyCa® (HA+CaHa), Radiesse® (CaHa), and Sculptra® (PLLA). Hydration and wound healing assays were also performed. Biomarker analysis focused on collagen types I, III, IV, VI, elastin, and cytokines (TGF-β1, IL-1β, IL-6, IL-10, TNFα).
Results: Rheologically, HA + DX displayed a balanced elastic and viscous profile, supporting its structural integrity and adaptability in dynamic facial areas. HA + DX demonstrated high stimulation of collagen types I, III, IV, VI, and elastin production, with enhanced fibroblast migration and wound healing at 72 hours compared to other fillers. HA + DX also modulated cytokines, increasing TGF-β1 and IL-10 while reducing TNFα and IL-1β, suggesting anti-inflammatory and regenerative properties.
Conclusions: HA + DX exhibited considerable benefits in collagen stimulation, wound healing, and cytokine modulation. Its favorable rheological characteristics and regenerative potential make it a promising option for facial rejuvenation and tissue regeneration.
No level assigned: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
期刊介绍：
Aesthetic Plastic Surgery is a publication of the International Society of Aesthetic Plastic Surgery and the official journal of the European Association of Societies of Aesthetic Plastic Surgery (EASAPS), Società Italiana di Chirurgia Plastica Ricostruttiva ed Estetica (SICPRE), Vereinigung der Deutschen Aesthetisch Plastischen Chirurgen (VDAPC), the Romanian Aesthetic Surgery Society (RASS), Asociación Española de Cirugía Estética Plástica (AECEP), La Sociedad Argentina de Cirugía Plástica, Estética y Reparadora (SACPER), the Rhinoplasty Society of Europe (RSE), the Iranian Society of Plastic and Aesthetic Surgeons (ISPAS), the Singapore Association of Plastic Surgeons (SAPS), the Australasian Society of Aesthetic Plastic Surgeons (ASAPS), the Egyptian Society of Plastic and Reconstructive Surgeons (ESPRS), and the Sociedad Chilena de Cirugía Plástica, Reconstructiva y Estética (SCCP).
Aesthetic Plastic Surgery provides a forum for original articles advancing the art of aesthetic plastic surgery. Many describe surgical craftsmanship; others deal with complications in surgical procedures and methods by which to treat or avoid them. Coverage includes "second thoughts" on established techniques, which might be abandoned, modified, or improved. Also included are case histories; improvements in surgical instruments, pharmaceuticals, and operating room equipment; and discussions of problems such as the role of psychosocial factors in the doctor-patient and the patient-public interrelationships.
Aesthetic Plastic Surgery is covered in Current Contents/Clinical Medicine, SciSearch, Research Alert, Index Medicus-Medline, and Excerpta Medica/Embase.
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