Uxia Gato-Diaz, Sandra Blanco-Garcia, Diana Peixoto, Angel Concheiro, Carmen Alvarez-Lorenzo, Barbara Blanco-Fernandez
{"title":"使用胶原-明胶-甲基丙烯酰胺-海藻酸盐生物墨水模拟促结缔组织微环境的3D生物打印胰腺癌模型。","authors":"Uxia Gato-Diaz, Sandra Blanco-Garcia, Diana Peixoto, Angel Concheiro, Carmen Alvarez-Lorenzo, Barbara Blanco-Fernandez","doi":"10.1021/acs.biomac.5c00450","DOIUrl":null,"url":null,"abstract":"<p><p>Pancreatic ductal adenocarcinoma is one of the cancers with the least favorable survival prognosis worldwide. It is characterized by a high desmoplastic stroma rich in collagen I, which regulates pancreatic cancer cells' behavior. There is a critical need to develop desmoplastic 3D models for preclinical testing. In this study, bioinks that imitate the biochemical characteristics of pancreatic ductal adenocarcinoma were developed to observe the influence that the desmoplastic extracellular matrix has on cancer cells. The bioinks were made of gelatin methacrylamide, alginate, and different concentrations of collagen I. Cancer cells were able to proliferate in all bioinks, presenting high paclitaxel resistance and a high expression of desmoplasia and extracellular matrix remodeling markers. The designed bioinks can play a crucial role in developing more clinically relevant cancer models for chemotherapeutic drug screening. Furthermore, they have significant potential for studying the influence of desmoplasia and for improving advanced treatment approaches for pancreatic cancer.</p>","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4000,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A 3D Bioprinted Pancreatic Cancer Model Using Collagen-Gelatin Methacrylamide-Alginate Bioinks to Mimic the Desmoplastic Microenvironment.\",\"authors\":\"Uxia Gato-Diaz, Sandra Blanco-Garcia, Diana Peixoto, Angel Concheiro, Carmen Alvarez-Lorenzo, Barbara Blanco-Fernandez\",\"doi\":\"10.1021/acs.biomac.5c00450\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Pancreatic ductal adenocarcinoma is one of the cancers with the least favorable survival prognosis worldwide. It is characterized by a high desmoplastic stroma rich in collagen I, which regulates pancreatic cancer cells' behavior. There is a critical need to develop desmoplastic 3D models for preclinical testing. In this study, bioinks that imitate the biochemical characteristics of pancreatic ductal adenocarcinoma were developed to observe the influence that the desmoplastic extracellular matrix has on cancer cells. The bioinks were made of gelatin methacrylamide, alginate, and different concentrations of collagen I. Cancer cells were able to proliferate in all bioinks, presenting high paclitaxel resistance and a high expression of desmoplasia and extracellular matrix remodeling markers. The designed bioinks can play a crucial role in developing more clinically relevant cancer models for chemotherapeutic drug screening. Furthermore, they have significant potential for studying the influence of desmoplasia and for improving advanced treatment approaches for pancreatic cancer.</p>\",\"PeriodicalId\":30,\"journal\":{\"name\":\"Biomacromolecules\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2025-10-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomacromolecules\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.biomac.5c00450\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomacromolecules","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.biomac.5c00450","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
A 3D Bioprinted Pancreatic Cancer Model Using Collagen-Gelatin Methacrylamide-Alginate Bioinks to Mimic the Desmoplastic Microenvironment.
Pancreatic ductal adenocarcinoma is one of the cancers with the least favorable survival prognosis worldwide. It is characterized by a high desmoplastic stroma rich in collagen I, which regulates pancreatic cancer cells' behavior. There is a critical need to develop desmoplastic 3D models for preclinical testing. In this study, bioinks that imitate the biochemical characteristics of pancreatic ductal adenocarcinoma were developed to observe the influence that the desmoplastic extracellular matrix has on cancer cells. The bioinks were made of gelatin methacrylamide, alginate, and different concentrations of collagen I. Cancer cells were able to proliferate in all bioinks, presenting high paclitaxel resistance and a high expression of desmoplasia and extracellular matrix remodeling markers. The designed bioinks can play a crucial role in developing more clinically relevant cancer models for chemotherapeutic drug screening. Furthermore, they have significant potential for studying the influence of desmoplasia and for improving advanced treatment approaches for pancreatic cancer.
期刊介绍：
Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine.
Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.
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