EmMYB5基因在枣仁类黄酮生物合成中的筛选与验证

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-10-14 DOI:10.1016/j.indcrop.2025.122113

Yuqi Feng, Jia Song, Dongsheng Wang, Xiaoyan Hu, Haoyang Li, Shengji Wang, Kai Zhao, Jing Wu, Zhaoshan Wang, Shuhui Du

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引用次数: 0

摘要

黄酮类化合物是天然的生物活性化合物，对人体健康有益。油棕是一种新兴的木本油料作物，油棕中的黄酮类化合物越来越受到人们的关注。然而，黄酮类化合物的组成、含量和生物合成机制仍不清楚，黄酮类化合物是黄酮类化合物的主要应用部分。本研究采用定量和多组学方法探讨了EMF中黄酮类化合物的动态和生物合成机制。9个收获时段（EMF1-EMF9）的黄酮类化合物含量变化在8.062 ~ 24.736 mg/g之间，在EMF2达到峰值。多组学整合鉴定出59个核心类黄酮生物合成基因，711个dam和7312个DEGs。富集分析强调了类黄酮、黄酮和黄烷醇的生物合成途径。WGCNA分析表明，MEblue与黄酮类化合物的数量有显著的相关性，该模块中的DEGs在次生代谢产物的生物合成途径中显著富集。相关网络鉴定出EmMYB5和EmMYB105是关键调控因子。总之，通过瞬时过表达和VIGS实验的功能表征表明，EmMYB5在EMF中作为类黄酮生物合成的负调节因子，酵母- 1杂交证实EmMYB5与Em4CL启动子结合。本研究的开展，揭示了EMF中黄酮类化合物生物合成的潜在机制，为进一步开发软霉生物强化功能食品奠定了基础。

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Screening and verification of EmMYB5 in flavonoids biosynthesis in Elaeagnus mollis dies kernel

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Screening and verification of EmMYB5 in flavonoids biosynthesis in Elaeagnus mollis dies kernel

Flavonoids are natural bioactive compounds with demonstrated health benefits in humans. Elaeagnus mollis Diels is a novel developing woody oil crop, and the flavonoids in E. mollis have attracted more attention. However, the composition content and biosynthesis mechanism of flavonoids in E. mollis kernel (EMF), the major application part, are still unclear. In this research, quantitative and multi-omics methods were orchestrated to explore dynamic and biosynthesis mechanism of flavonoids in EMF. The flavonoids content in EMF at nine harvest times (EMF1-EMF9) varied between 8.062 and 24.736 mg/g, peaking at EMF2. Multi-omics integration identified 59 core flavonoids biosynthesis genes, 711 DAMs, and 7312 DEGs. Enrichment analysis highlighted flavonoid, flavone and flavanol biosynthesis pathways. WGCNA analysis illustrated that the MEblue showed remarkable association with flavonoids quantity, and DEGs in this module significantly enriched in biosynthesis of secondary metabolites pathway. Correlation networks identified EmMYB5 and EmMYB105 as key regulators. Collectively, the functional characterization through transient overexpression and VIGS assays demonstrated that EmMYB5 acted as a negative regulator of flavonoids biosynthesis in EMF, with yeast-one-hybrid confirming EmMYB5 binding to the Em4CL promoter. The implementation of this study dissects the underlying mechanism of flavonoids biosynthesis in EMF, and also lays the foundation for further biofortified functional foods development of E. mollis.

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.