Alfredo Morales, Peiyong Ma, ZhaoDong Jia, Dania Rodríguez, Iván Javier Pastrana Vargas, Rosa Elena González, Osmany Molina, Alay Jiménez, Yuniel Rodríguez, Lilian Morales, Yoel Beovides, Orelvis Portal, Xiaofeng Bian
{"title":"密缕草表型特征的解码。全球甘薯（Ipomoea batatas）抗性的研究[j]。[林].)种质资源收集。","authors":"Alfredo Morales, Peiyong Ma, ZhaoDong Jia, Dania Rodríguez, Iván Javier Pastrana Vargas, Rosa Elena González, Osmany Molina, Alay Jiménez, Yuniel Rodríguez, Lilian Morales, Yoel Beovides, Orelvis Portal, Xiaofeng Bian","doi":"10.3389/fpls.2025.1625810","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Sweetpotato (<i>Ipomoea batatas</i> [L.] Lam.) is a critical global food crop that suffers devastating yield losses from the sweetpotato weevil (<i>Cylas formicarius</i>), especially in tropical regions where chemical control is often impractical. Breeding for stable resistance has been hindered by an insufficient characterization of reliable phenotypic markers across diverse genetic backgrounds.</p><p><strong>Methods: </strong>We evaluated 731 accessions from Cuba's national sweetpotato collection, enriched with global varieties, to identify morphological traits associated with natural resistance to <i>C. formicarius</i>. Resistance and susceptibility were assessed through combined field and laboratory bioassays.</p><p><strong>Results: </strong>Only 6.5% of the accessions demonstrated resistance (<10% infestation), while 80% were highly susceptible. Weak to moderate correlations linked resistance to smoother root surfaces (r = 0.31) and lighter flesh pigmentation (r = -0.38). The strongest correlation was observed with deeper tuberization (r = -0.72). Six Cuban genotypes combined agronomic viability (yield >10 t ha⁻¹) with resistance. Five of these employed deep tuberization as a physical escape mechanism, while one genotype, INIVIT B-25, exhibited shallow tuberization (mean depth 4.53 cm) yet maintained resistance, suggesting a biochemical defense strategy. Under controlled infestation, INIVIT B-2022 demonstrated the strongest antibiosis effect, suppressing adult emergence to just two individuals.</p><p><strong>Discussion: </strong>Our study decodes key phenotypic signatures of weevil resistance, providing immediately actionable morphological traits for use in Caribbean breeding programs. The discovery of a resistant genotype with shallow roots indicates the presence of a non-escape, potentially biochemical resistance mechanism. This highlights the critical need for subsequent molecular studies to uncover the complementary genetic and biochemical bases of these defenses.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1625810"},"PeriodicalIF":4.1000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12511093/pdf/","citationCount":"0","resultStr":"{\"title\":\"Decoding phenotypic signatures of <i>Cylas formicarius</i> Fab. resistance in a global sweetpotato (<i>Ipomoea batatas</i> [L.] Lam.) germplasm collection.\",\"authors\":\"Alfredo Morales, Peiyong Ma, ZhaoDong Jia, Dania Rodríguez, Iván Javier Pastrana Vargas, Rosa Elena González, Osmany Molina, Alay Jiménez, Yuniel Rodríguez, Lilian Morales, Yoel Beovides, Orelvis Portal, Xiaofeng Bian\",\"doi\":\"10.3389/fpls.2025.1625810\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Sweetpotato (<i>Ipomoea batatas</i> [L.] Lam.) is a critical global food crop that suffers devastating yield losses from the sweetpotato weevil (<i>Cylas formicarius</i>), especially in tropical regions where chemical control is often impractical. Breeding for stable resistance has been hindered by an insufficient characterization of reliable phenotypic markers across diverse genetic backgrounds.</p><p><strong>Methods: </strong>We evaluated 731 accessions from Cuba's national sweetpotato collection, enriched with global varieties, to identify morphological traits associated with natural resistance to <i>C. formicarius</i>. Resistance and susceptibility were assessed through combined field and laboratory bioassays.</p><p><strong>Results: </strong>Only 6.5% of the accessions demonstrated resistance (<10% infestation), while 80% were highly susceptible. Weak to moderate correlations linked resistance to smoother root surfaces (r = 0.31) and lighter flesh pigmentation (r = -0.38). The strongest correlation was observed with deeper tuberization (r = -0.72). Six Cuban genotypes combined agronomic viability (yield >10 t ha⁻¹) with resistance. Five of these employed deep tuberization as a physical escape mechanism, while one genotype, INIVIT B-25, exhibited shallow tuberization (mean depth 4.53 cm) yet maintained resistance, suggesting a biochemical defense strategy. Under controlled infestation, INIVIT B-2022 demonstrated the strongest antibiosis effect, suppressing adult emergence to just two individuals.</p><p><strong>Discussion: </strong>Our study decodes key phenotypic signatures of weevil resistance, providing immediately actionable morphological traits for use in Caribbean breeding programs. The discovery of a resistant genotype with shallow roots indicates the presence of a non-escape, potentially biochemical resistance mechanism. 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Decoding phenotypic signatures of Cylas formicarius Fab. resistance in a global sweetpotato (Ipomoea batatas [L.] Lam.) germplasm collection.
Introduction: Sweetpotato (Ipomoea batatas [L.] Lam.) is a critical global food crop that suffers devastating yield losses from the sweetpotato weevil (Cylas formicarius), especially in tropical regions where chemical control is often impractical. Breeding for stable resistance has been hindered by an insufficient characterization of reliable phenotypic markers across diverse genetic backgrounds.
Methods: We evaluated 731 accessions from Cuba's national sweetpotato collection, enriched with global varieties, to identify morphological traits associated with natural resistance to C. formicarius. Resistance and susceptibility were assessed through combined field and laboratory bioassays.
Results: Only 6.5% of the accessions demonstrated resistance (<10% infestation), while 80% were highly susceptible. Weak to moderate correlations linked resistance to smoother root surfaces (r = 0.31) and lighter flesh pigmentation (r = -0.38). The strongest correlation was observed with deeper tuberization (r = -0.72). Six Cuban genotypes combined agronomic viability (yield >10 t ha⁻¹) with resistance. Five of these employed deep tuberization as a physical escape mechanism, while one genotype, INIVIT B-25, exhibited shallow tuberization (mean depth 4.53 cm) yet maintained resistance, suggesting a biochemical defense strategy. Under controlled infestation, INIVIT B-2022 demonstrated the strongest antibiosis effect, suppressing adult emergence to just two individuals.
Discussion: Our study decodes key phenotypic signatures of weevil resistance, providing immediately actionable morphological traits for use in Caribbean breeding programs. The discovery of a resistant genotype with shallow roots indicates the presence of a non-escape, potentially biochemical resistance mechanism. This highlights the critical need for subsequent molecular studies to uncover the complementary genetic and biochemical bases of these defenses.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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