Belhadj Oussama, Sahnouni Fatima, Hariri Ahmed, Souafi Kheira, Missoum Ilham
{"title":"甜菜根粉新型微生物培养基的配方：一种可持续替代商业Man-de-Rogosa Sharp琼脂（MRS）培养乳酸菌的培养基。","authors":"Belhadj Oussama, Sahnouni Fatima, Hariri Ahmed, Souafi Kheira, Missoum Ilham","doi":"10.1007/s11274-025-04561-x","DOIUrl":null,"url":null,"abstract":"<p><p>This preliminary study investigates the feasibility of utilizing Algerian beetroot as a sustainable alternative to commercial Man-de-Rogosa Sharp Agar (MRS) for cultivating lactic acid bacteria. The research explores the optimization of microbial media formulations incorporating beetroot powder and assesses its efficacy in supporting the growth of Lactobacillus plantarum, Lactobacillus fermentum, and Enterococcus durans. Methodologically, vegetal samples were harvested and dried, bacterial strains prepared, and phytochemical analyses conducted. Titratable acidity and sugar content in the beet-based medium were measured, revealing pH 5.5, 13% titratable acidity, and 67 g/L sugar content. Results indicate that while beetroot juice alone exhibited limited bacterial growth due to its inadequate nutritional properties, formulations incorporating additives such as magnesium sulfate, ammonium sulfate, and di-potassium phosphate showed improved growth. Particularly, the formulation \"BJFA-SM\" (with manganese sulfate) demonstrated comparable growth to MRS, with reduced generation times for L. fermentum and E. durans. Additionally, the disappearance of betalain pigment from beetroot-based media indicates successful bacterial growth. Agar plate testing has revealed similar macroscopic characteristics and cell counts to the MRS medium indicating the potential of beetroot-based media as a sustainable substitute for traditional growth media. Finally, the BJFA-MS medium showed superior growth for L. plantarum, E. durans, and L. fermentum compared to MRS, with faster growth rates. However, it exhibited lower acid production efficiency than MRS. This study underscores the viability of beetroot-derived formulations as eco-friendly alternatives for cultivating lactic acid bacteria, with implications for sustainable microbiology research and biotechnological applications.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 10","pages":"372"},"PeriodicalIF":4.2000,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Formulations of novel microbial medium from beetroot powder: a sustainable substitute for commercial Man-de-Rogosa Sharp agar (MRS) in cultivating lactic acid bacteria.\",\"authors\":\"Belhadj Oussama, Sahnouni Fatima, Hariri Ahmed, Souafi Kheira, Missoum Ilham\",\"doi\":\"10.1007/s11274-025-04561-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This preliminary study investigates the feasibility of utilizing Algerian beetroot as a sustainable alternative to commercial Man-de-Rogosa Sharp Agar (MRS) for cultivating lactic acid bacteria. The research explores the optimization of microbial media formulations incorporating beetroot powder and assesses its efficacy in supporting the growth of Lactobacillus plantarum, Lactobacillus fermentum, and Enterococcus durans. Methodologically, vegetal samples were harvested and dried, bacterial strains prepared, and phytochemical analyses conducted. Titratable acidity and sugar content in the beet-based medium were measured, revealing pH 5.5, 13% titratable acidity, and 67 g/L sugar content. Results indicate that while beetroot juice alone exhibited limited bacterial growth due to its inadequate nutritional properties, formulations incorporating additives such as magnesium sulfate, ammonium sulfate, and di-potassium phosphate showed improved growth. Particularly, the formulation \\\"BJFA-SM\\\" (with manganese sulfate) demonstrated comparable growth to MRS, with reduced generation times for L. fermentum and E. durans. Additionally, the disappearance of betalain pigment from beetroot-based media indicates successful bacterial growth. Agar plate testing has revealed similar macroscopic characteristics and cell counts to the MRS medium indicating the potential of beetroot-based media as a sustainable substitute for traditional growth media. Finally, the BJFA-MS medium showed superior growth for L. plantarum, E. durans, and L. fermentum compared to MRS, with faster growth rates. However, it exhibited lower acid production efficiency than MRS. 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Formulations of novel microbial medium from beetroot powder: a sustainable substitute for commercial Man-de-Rogosa Sharp agar (MRS) in cultivating lactic acid bacteria.
This preliminary study investigates the feasibility of utilizing Algerian beetroot as a sustainable alternative to commercial Man-de-Rogosa Sharp Agar (MRS) for cultivating lactic acid bacteria. The research explores the optimization of microbial media formulations incorporating beetroot powder and assesses its efficacy in supporting the growth of Lactobacillus plantarum, Lactobacillus fermentum, and Enterococcus durans. Methodologically, vegetal samples were harvested and dried, bacterial strains prepared, and phytochemical analyses conducted. Titratable acidity and sugar content in the beet-based medium were measured, revealing pH 5.5, 13% titratable acidity, and 67 g/L sugar content. Results indicate that while beetroot juice alone exhibited limited bacterial growth due to its inadequate nutritional properties, formulations incorporating additives such as magnesium sulfate, ammonium sulfate, and di-potassium phosphate showed improved growth. Particularly, the formulation "BJFA-SM" (with manganese sulfate) demonstrated comparable growth to MRS, with reduced generation times for L. fermentum and E. durans. Additionally, the disappearance of betalain pigment from beetroot-based media indicates successful bacterial growth. Agar plate testing has revealed similar macroscopic characteristics and cell counts to the MRS medium indicating the potential of beetroot-based media as a sustainable substitute for traditional growth media. Finally, the BJFA-MS medium showed superior growth for L. plantarum, E. durans, and L. fermentum compared to MRS, with faster growth rates. However, it exhibited lower acid production efficiency than MRS. This study underscores the viability of beetroot-derived formulations as eco-friendly alternatives for cultivating lactic acid bacteria, with implications for sustainable microbiology research and biotechnological applications.
期刊介绍：
World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology.
Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions.
Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories:
· Virology
· Simple isolation of microbes from local sources
· Simple descriptions of an environment or reports on a procedure
· Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism
· Data reporting on host response to microbes
· Optimization of a procedure
· Description of the biological effects of not fully identified compounds or undefined extracts of natural origin
· Data on not fully purified enzymes or procedures in which they are applied
All articles published in the Journal are independently refereed.
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