Simulated dose-dependent effects of 660 nm laser photostimulation on human sperm motility and acrosome integrity using MATLAB modeling.

Low-level laser therapy (LLLT) has been demonstrated to have the potential for enhancing sperm function by means of photobiomodulation. Therapeutic dosing parameters to maximize the effect, though, remain undefined. To simulate dose-dependent effects of 660 nm laser irradiation on human sperm motility and acrosome integrity using MATLAB-based modeling. A simulation was established on the computer to assess reactions of sperm over a fluence range of 0.15-2.4 J/cm², which was produced from varying power densities (5-20 mW/cm²) and durations (30-120 s). Logistic and Gaussian functions with biologically natural noise were used in modeling motility and acrosome integrity in order to mimic experimental variation. Motility increased in sigmoidal manner, peaking at around 1.2 J/cm², while acrosome integrity peaked at around 0.6 J/cm² in the form of a bell curve. Optimal fluence range (0.6-0.9 J/cm²) balanced both improving motility and maintaining acrosomes intact. 660 nm laser stimulation of sperm motility and acrosome integrity is enhanced within a narrow fluence window. This in silico process offers a predictive, reproducible method for the determination of optimal PBM parameters in reproductive application.