利用微珠上转换纳米颗粒检测亚飞摩尔浓度MicroRNA的移液管尖端即时检测平台

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-10-10 DOI:10.1021/acs.nanolett.5c02759

Mohammad Sadraeian, Jiajia Zhou, Jiayan Liao, Jawairia Umar Khan, Mahnaz Maddahfar, Yuen Yee Cheng, Milad Sabbagh, Dayong Jin

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引用次数: 0

摘要

microrna是有效的早期疾病生物标志物，但适用于即时检测（POCT）的超灵敏检测方法仍然很少。我们提出了一种用于定量检测致癌miR-27a的移液器平台。该方法采用夹心杂交策略，利用微珠上的生物素化捕获寡核苷酸和上转换纳米颗粒（UCNP）共轭寡核苷酸作为报告探针。加入高掺杂的UCNPs以提高检测灵敏度。珠子-寡核苷酸复合物在移液管尖端流体中的动态迁移率显著提高了杂交效率和信号检测特异性。该分析从128原子摩尔（aM）到2.05 fM呈线性，达到158原子摩尔的检测极限（LOD），并且比不匹配的链产生33倍的信号优势。我们的平台为检测低丰度mirna提供了一种简单、快速、经济的解决方案，并具有功能化mirna珠的可重复使用性。它的可扩展性和最小的仪器要求使其成为先进分子诊断和POCT应用的有力候选者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A Pipette-Tip-Based Point-of-Care Test Platform for Detection of MicroRNA at Subfemtomolar Concentrations Using Upconverting Nanoparticles on Microbeads

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A Pipette-Tip-Based Point-of-Care Test Platform for Detection of MicroRNA at Subfemtomolar Concentrations Using Upconverting Nanoparticles on Microbeads

MicroRNAs are potent early disease biomarkers, yet ultrasensitive assays suitable for point-of-care testing (POCT) remain scarce. We present a pipet-tip platform for quantitative detection of oncogenic miR-27a. This method employs a sandwich hybridization strategy utilizing biotinylated capture oligos on microbeads and upconversion nanoparticle (UCNP)-conjugated oligos as the reporting probe. Highly doped UCNPs were incorporated to enhance the detection sensitivity. The dynamic mobility of the bead-oligo complexes within the pipet tip fluid significantly improves the hybridization efficiency and signal detection specificity. The assay is linear from 128 attomolar (aM) to 2.05 fM, achieves a 158 aM limit of detection (LOD), and yields a 33-fold signal advantage over mismatched strands. Our platform offers a simplified, rapid, and cost-effective solution for detecting low-abundance miRNAs with the reusability of functionalized miRNA-beads. Its scalability and minimal instrumentation requirements make it a strong candidate for advanced molecular diagnostics and POCT applications.

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.